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Titolo:
Cloning and sequence analysis of cDNAs encoding the heavy and light chain variable regions of an Ab2 beta anti-idiotypic monoclonal antibody possessing an internal image of cocaine
Autore:
Ho, M; Segre, M;
Indirizzi:
Univ Illinois, Dept Pathobiol, Urbana, IL 61802 USA Univ Illinois Urbana IL USA 61802 s, Dept Pathobiol, Urbana, IL 61802 USA
Titolo Testata:
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION
fascicolo: 1-3, volume: 1521, anno: 2001,
pagine: 135 - 140
SICI:
0167-4781(20011031)1521:1-3<135:CASAOC>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN DOPAMINE TRANSPORTER; ANTIIDIOTYPIC ANTIBODIES; MOLECULAR-BASIS; GENES; MOUSE; SUBSTRATE; SUBGROUP; MIMICRY; AB1;
Keywords:
anti-idiotypic monoclonal antibody; dopamine transporter; cocaine; nucleotide sequence;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Segre, M Univ Illinois, Dept Pathobiol, 2001 S Lincoln Ave, Urbana, IL 61802 USA Univ Illinois 2001 S Lincoln Ave Urbana IL USA 61802 IL 61802 USA
Citazione:
M. Ho e M. Segre, "Cloning and sequence analysis of cDNAs encoding the heavy and light chain variable regions of an Ab2 beta anti-idiotypic monoclonal antibody possessing an internal image of cocaine", BBA-GENE ST, 1521(1-3), 2001, pp. 135-140

Abstract

We report here the cloning and sequence analysis of cDNAs encoding he variable regions of an Ab2 beta anti-idiotypic monoclonal antibody (K1-4c, gamma1 kappa) that mimics the configuration of cocaine. The Ab2 beta specifically binds to the human dopamine transporter as shown by confocal immunofluorescence microscopy. The sequence of the heavy chain complementarity-determining region 3 of K1-4c is strikingly similar to that of a monoclonal antibody (F11.2.32) specific for HIV-1 protease. Three or four amino acids in theepitope recognized by the anti-HIV-1 protease antibody are also present inthe third extracellular loop of the dopamine transporter, This epitope is within the conserved region of the known transporters for dopamine, norepinephrine and serotonin in Homo sapiens, Rattus norvegicus, Caenorhabditis elegans and Drosophila melanogaster. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/07/20 alle ore 05:50:24