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Titolo:
Pulsatile flow regulates monocyte adhesion to oxidized lipid-induced endothelial cells
Autore:
Hsiai, TK; Cho, SK; Reddy, S; Hama, S; Navab, M; Demer, LL; Honda, HM; Ho, CM;
Indirizzi:
Univ Calif Los Angeles, Sch Med, Div Cardiol, Dept Med, Los Angeles, CA 90095 USA Univ Calif Los Angeles Los Angeles CA USA 90095 Los Angeles, CA 90095 USA Univ Calif Los Angeles, Sch Med, Dept Physiol, Los Angeles, CA 90095 USA Univ Calif Los Angeles Los Angeles CA USA 90095 Los Angeles, CA 90095 USA Univ Calif Los Angeles, Sch Med, Dept Mol & Med Pharmacol, Los Angeles, CA90095 USA Univ Calif Los Angeles Los Angeles CA USA 90095 Los Angeles, CA90095 USA Univ Calif Los Angeles, Sch Engn & Appl Sci, Dept Mech & Aerosp Engn, Los Angeles, CA 90024 USA Univ Calif Los Angeles Los Angeles CA USA 90024 Los Angeles, CA 90024 USA
Titolo Testata:
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
fascicolo: 11, volume: 21, anno: 2001,
pagine: 1770 - 1776
SICI:
1079-5642(200111)21:11<1770:PFRMAT>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
LOW-DENSITY-LIPOPROTEIN; SHEAR-STRESS GRADIENTS; NITRIC-OXIDE SYNTHASE; ATHEROSCLEROTIC LESIONS; CHEMOTACTIC PROTEIN-1; VASCULAR ENDOTHELIUM; CAROTID BIFURCATION; NONHUMAN PRIMATE; STEADY SHEAR; FOAM CELLS;
Keywords:
shear stress; slew rate; oxidized 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphorylcholine; monocyte adhesion; monocyte chemoattractant protein-1;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
54
Recensione:
Indirizzi per estratti:
Indirizzo: Hsiai, TK Univ Calif Los Angeles, Sch Med, Div Cardiol, Dept Med, 47-123 CHS, Los Angeles, CA 90095 USA Univ Calif Los Angeles 47-123 CHS Los AngelesCA USA 90095 5 USA
Citazione:
T.K. Hsiai et al., "Pulsatile flow regulates monocyte adhesion to oxidized lipid-induced endothelial cells", ART THROM V, 21(11), 2001, pp. 1770-1776

Abstract

Oxidized 1-palmitoyl-2-aracbidonoyl-sn-glycero-3-phosphorylcholine (ox-PAPC), a component of minimally modified low density lipoprotein, induces monocyte adhesion to endothelial cells. It is not known whether the upstroke slopes of pulsatile flow, defined as shear stress slew rates (partial derivative tau/partial derivativet), can regulate monocyte binding to ox-PAPC-treated bovine aortic endothelial cells (BAECs). At 60 cycles per minute, ox-PAPC-treated BAECs were exposed to 3 conditions representing known vascular conditions: (1) high shear stress slew rate (partial derivative tau/partial derivativet = 293 dyne. cm(-2) (.) s(-1)), with time-averaged shear stress = 50 dyne/cm(2); (2) low shear stress slew rate (partial derivative tau/partial derivativet = 71 dyne (.) cm(-2 .) s(-1)), with identical time-averaged shear stress; and (3) reversing oscillating flow (0 +/- 2.6 mm Hg). Reverse transcription-polymerase chain reaction and quantification were performed for monocyte chemoattractant protein-1 (MCP-1) mRNA expression. High partial derivative tau/partial derivativet reduced monocyte binding to ox-PAPC-treated BAECs by 64 +/- 3.2% compared with static conditions, and low partial derivative tau/partial derivativet reduced monocyte binding by 31 +/- 3.4%, whereas oscillating flow increased monocyte binding by 22 +/- 1.7% (P <0.005). High partial derivative tau/partial derivativet downregulated MCP-1 expression by 33 +/- 8%, and low partial derivative tau/partial derivativet downregulated MCP-1 expression by 15 +/- 4%, but oscillating flow upregulated MCP-1 by 13 +/- 5%. These results suggest that shear stress slew rates regulate monocyte binding by modulating the expression of a potent monocyte chemoattractant.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/09/20 alle ore 23:31:42