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Titolo:
Induction of nuclear orphan receptor NGFI-B gene and apoptosis in rat vascular smooth muscle cells treated with pyrrolidinedithiocarbamate
Autore:
Watanabe, T; Yoshizumi, M; Akishita, M; Eto, M; Toba, K; Hashimoto, M; Nagano, K; Liang, YQ; Ohike, Y; Iijima, K; Sudoh, N; Kim, S; Nakaoka, T; Yamashita, N; Ako, J; Ouchi, Y;
Indirizzi:
Univ Tokyo, Grad Sch Med, Dept Geriatr Med, Bunkyo Ku, Tokyo 1138655, Japan Univ Tokyo Tokyo Japan 1138655 iatr Med, Bunkyo Ku, Tokyo 1138655, Japan Univ Tokyo, Inst Med Sci, Dept Adv Med, Bunkyo Ku, Tokyo 1138655, Japan Univ Tokyo Tokyo Japan 1138655 Adv Med, Bunkyo Ku, Tokyo 1138655, Japan
Titolo Testata:
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
fascicolo: 11, volume: 21, anno: 2001,
pagine: 1738 - 1744
SICI:
1079-5642(200111)21:11<1738:IONORN>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
NF-KAPPA-B; DIFFERENTIAL REGULATION; EXPRESSION; GROWTH; NUR77; SUPERFAMILY; CULTURE; BRAIN; TRANSCRIPTION; PROLIFERATION;
Keywords:
vascular smooth muscle cells; apoptosis; nuclear receptor; cell density;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Ouchi, Y Univ Tokyo, Grad Sch Med, Dept Geriatr Med, Bunkyo Ku, 7-3-1 Hongo, Tokyo 1138655, Japan Univ Tokyo 7-3-1 Hongo Tokyo Japan 1138655 Tokyo 1138655, Japan
Citazione:
T. Watanabe et al., "Induction of nuclear orphan receptor NGFI-B gene and apoptosis in rat vascular smooth muscle cells treated with pyrrolidinedithiocarbamate", ART THROM V, 21(11), 2001, pp. 1738-1744

Abstract

NGFI-B is one of the orphan nuclear receptors, and its gene is implicated in the apoptosis of T cells. The aim of this study was to investigate the expression and the role of NGFI-B in vascular smooth muscle cells (VSMCs). Pyrrolidinedithiocarbamate (PDTC) is a modulator of an oxidative state and is reported to induce apoptosis only when the density of VSMCs is low. Underlow VSMC density (10000 cells/cm(2)), addition of PDTC (0.1 to 10 mu mol/L) caused apoptosis of VSMCs, which was confirmed by Hoechst 33258 staining under fluorescence microscopy. At low VSMC density, expression of NGFI-B mRNA was induced 1 hour after the addition of PDTC, pealing at 6 hours, and persisted for up to 12 hours. The protein level of NGFI-B was increased 4 hours after PDTC addition and persisted for up to 12 hours. Under low VSMC density, PDTC-induced expression of NGFI-B mRNA was correlated with the magnitude of apoptosis, which was quantified by enzyme immunoassay for histone-associated DNA fragments. In contrast, when the density of VSMCs was high (50000 cells/cm(2)), PDTC did not induce apoptosis, and the expression of NGFI-B was only transient. This transient expression pattern was also seen when VSMCs were treated with phorbol ester, calcium ionophore, hydrogen peroxide, or angiotensin II, even at low cell density. We next investigated whether the NGFI-B gene may act as a transcription factor under treatment with PDTC by measuring the promoter activity of luciferase reporter plasmids thatcontained typical NGFI-B-responsive elements. The PDTC-induced transcriptional activity of NGFI-B was 2-fold higher at low cell density than at high cell density. These data demonstrate that NGFI-B can be induced in VSMCs and suggest that NGFI-B may play a role in PDTC-induced VSMC apoptosis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/07/20 alle ore 01:44:38