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Titolo:
Olopatadine inhibits anti-immunoglobulin E-stimulated conjunctival mast cell upregulation of ICAM-1 expression on conjunctival epithelial cells
Autore:
Cook, EB; Stahl, JL; Barney, NP; Graziano, FM;
Indirizzi:
Univ Wisconsin, Sch Med, Dept Med, Madison, WI USA Univ Wisconsin MadisonWI USA consin, Sch Med, Dept Med, Madison, WI USA Univ Wisconsin, Sch Med, Dept Ophthalmol & Visual Sci, Madison, WI USA Univ Wisconsin Madison WI USA t Ophthalmol & Visual Sci, Madison, WI USA
Titolo Testata:
ANNALS OF ALLERGY ASTHMA & IMMUNOLOGY
fascicolo: 5, volume: 87, anno: 2001,
pagine: 424 - 429
SICI:
1081-1206(200111)87:5<424:OIAECM>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTERCELLULAR-ADHESION MOLECULE-1; TYROSINE PHOSPHORYLATION; INTERFERON-GAMMA; ACTIVATION; ALPHA; MONOLAYERS; CYTOKINES; SKIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Graziano, FM Univ Wisconsin Hosp & Clin, Clin Sci Ctr H6 368, 600 HighlandAve, Madison, WI 53792 USA Univ Wisconsin Hosp & Clin 600 Highland Ave Madison WI USA 53792
Citazione:
E.B. Cook et al., "Olopatadine inhibits anti-immunoglobulin E-stimulated conjunctival mast cell upregulation of ICAM-1 expression on conjunctival epithelial cells", ANN ALLER A, 87(5), 2001, pp. 424-429

Abstract

Background: Olopatadine is a clinically effective dual-action (antihistamine/mast cell stabilizer) ophthalmic antiallergic agent. We have previously demonstrated that olopatadine inhibits tumor necrosis factor alpha (TNF-alpha) release from purified human conjunctival mast cells and that supemates from stimulated mast cells upregulate intercellular adhesion molecule 1 (ICAM-1) expression on epithelial cells via TNF-alpha. Objective: To investigate the effect of olopatadine on the TNF-alpha -mediated mast cell upregulation of ICAM-1 expression on conjunctival epithelialcells. Methods: Human conjunctival mast cells and epithelial cells were purified (> 95%) from cadaveric tissue. Conjunctival mast cells were preincubated with three doses (30, 300, or 3,000 muM) of olopatadine or buffer alone for 30 minutes followed by 90-minute challenge with anti-immunoglobulin E (10 mug/mL). The resulting supernates were incubated with conjunctival epithelialcell monolayers for 24 hours along with the following treatments: rTNF-alpha, mast cell supernate + anti-TNF-alpha, recombinant (r)TNF-alpha + anti-TNF-alpha, the three doses of olopatadine, olopatadine supernates, olopatadine supernates + rTNF-alpha. ICAM-1 expression was measured using flow cytometry. Results: Anti-IgE-stimulated human conjunctival mast cell supernates upregulated human conjunctival epithelial cell ICAM-1 expression to the same extent as rTNF-alpha. ICAM-1 upregulation could be completely blocked with anti-TNF-alpha. Preincubation of conjunctival mast cells with olopatadine significantly blocked the ability of supernates to upregulate ICAM-1 on conjunctival epithelial cells. ICAM-1 expression could be restored by adding rTNF-alpha to the olopatadine-preincubated mast cell supernates. Conclusions: Olopatadine is able to significantly decrease the anti-immunoglobulin E mast cell supernate-mediated upregulation of ICAM-1 on human conjunctival epithelial cells in vitro. This seems to be mediated through an effect on a TNF-aspecific mechanism.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/04/20 alle ore 22:50:10