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Titolo:
Detection of Leishmania causing visceral leishmaniasis in the Old and New Worlds by a polymerase chain reaction assay based on telomeric sequences
Autore:
Chiurillo, MA; Sachdeva, M; Dole, VS; Yepes, Y; Miliani, E; Vazquez, L; Rojas, A; Crisante, G; Guevara, P; Anez, N; Madhubala, R; Ramirez, JL;
Indirizzi:
Cent Univ Venezuela, Inst Expt Biol, Caracas 1041A, Venezuela Cent Univ Venezuela Caracas Venezuela 1041A ol, Caracas 1041A, Venezuela Inst Estudios Avanzados, Ctr Biotechnol, Caracas, Venezuela Inst Estudios Avanzados Caracas Venezuela iotechnol, Caracas, Venezuela Jawaharlal Nehru Univ, Sch Life Sci, New Delhi 110067, India Jawaharlal Nehru Univ New Delhi India 110067 ci, New Delhi 110067, India Univ Francis Miranda, Escuela Med, Coro, Venezuela Univ Francis Miranda Coro Venezuela randa, Escuela Med, Coro, Venezuela Univ Los Andes, Fac Med, Hosp Cent Valera, Hematol Serv, Valera, VenezuelaUniv Los Andes Valera Venezuela Valera, Hematol Serv, Valera, Venezuela Univ Los Andes, Fac Ciencias, Merida 5101, Venezuela Univ Los Andes Merida Venezuela 5101 ac Ciencias, Merida 5101, Venezuela
Titolo Testata:
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
fascicolo: 5, volume: 65, anno: 2001,
pagine: 573 - 582
SICI:
0002-9637(200111)65:5<573:DOLCVL>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
DNA; DONOVANI; IDENTIFICATION; AMPLIFICATION; PARASITES; HETEROGENEITY; COLOMBIENSIS; KINETOPLAST; GENOTYPES; DIAGNOSIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: Ramirez, JL Cent Univ Venezuela, Inst Expt Biol, Calle Suapure,Colina de Bello Monte, Caracas 1041A, Venezuela Cent Univ Venezuela Calle Suapure,Colina de Bello Monte Caracas Venezuela 1041A
Citazione:
M.A. Chiurillo et al., "Detection of Leishmania causing visceral leishmaniasis in the Old and New Worlds by a polymerase chain reaction assay based on telomeric sequences", AM J TROP M, 65(5), 2001, pp. 573-582

Abstract

We present a new polymerase chain reaction assay based on telomeric sequences of Leishmania donovani. When this assay was used in dilutions of purified L. donovani DNA, a strong amplification signal was observed with 1 fg ofDNA. In a specificity test that used purified DNA from Old World and New World Leishmania, the assay recognized all parasites isolated from patients with visceral leishmaniasis, except for 2 isolates of Leishmania colombiensis from Venezuela and 1 isolate from Brazil. All Leishmania major and Leishmania tropica isolates tested were negative, except for one isolate in eachspecies. We also used the assay on fresh and archive bone marrow samples recovered from Giemsa-stained slides and from dried blood stains.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 17:38:57