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Titolo:
Steroid sensitivity of norepinephrine uptake by human bronchial arterial and rabbit aortic smooth muscle cells
Autore:
Horvath, G; Lieb, T; Conner, GE; Salathe, M; Wanner, A;
Indirizzi:
Univ Miami, Sch Med, Div Pulm & Crit Care Med, Miami, FL 33101 USA Univ Miami Miami FL USA 33101 v Pulm & Crit Care Med, Miami, FL 33101 USA Univ Miami, Sch Med, Dept Cell Biol & Anat, Miami, FL 33101 USA Univ Miami Miami FL USA 33101 Dept Cell Biol & Anat, Miami, FL 33101 USA
Titolo Testata:
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
fascicolo: 4, volume: 25, anno: 2001,
pagine: 500 - 506
SICI:
1044-1549(200110)25:4<500:SSONUB>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
ORGANIC CATION TRANSPORTER; MUCOSAL BLOOD-FLOW; RAT-HEART; FUNCTIONAL-CHARACTERIZATION; SUBSTRATE-SPECIFICITY; CLONING; KIDNEY; EXPRESSION; CORTICOSTERONE; NORADRENALINE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
53
Recensione:
Indirizzi per estratti:
Indirizzo: Wanner, A Univ Miami, Sch Med, Div Pulm & Crit Care Med, POB 016960,R-47, Miami, FL 33101 USA Univ Miami POB 016960,R-47 Miami FL USA 33101 iami, FL 33101 USA
Citazione:
G. Horvath et al., "Steroid sensitivity of norepinephrine uptake by human bronchial arterial and rabbit aortic smooth muscle cells", AM J RESP C, 25(4), 2001, pp. 500-506

Abstract

We have shown that an inhaled glucocorticosteroid (GS) causes alpha (1)-adrenergic antagonist-blockable, rapid, and transient bronchial vasoconstriction in healthy and asthmatic subjects. Steroids inhibit norepinephrine (NE)uptake by non-neuronal cells, thereby increasing NE concentration at a-adrenergic receptor sites. This could explain the GS-induced bronchial vasoconstriction. We therefore studied expression of the steroid-sensitive extraneuronal monoamine transporter (EMT) and steroid sensitivity of NE uptake in human bronchial artery and rabbit aorta (as a substitute for the limited supply of human bronchial artery). NE uptake was measured using a semiquantitative, sucrose-potassium phosphate-glyoxylic acid fluorescence method that we newly adapted for use in single cells. Both human bronchial arteries andrabbit aorta expressed messenger RNA for EMT, and steroids blocked NE uptake into freshly dissociated human bronchial arterial and rabbit aortic smooth-muscle cells (SMCs). In the latter, inhibition of NE uptake by steroids was not altered, either by a protein synthesis inhibitor (cycloheximide) orby a transcription inhibitor (actinomycin D), and corticosterone made membrane-impermeant by conjugation to bovine serum albumin inhibited NE uptake equipotently. These data show that NE uptake into bronchial arterial and rabbit aortic SMCs is sensitive to steroids, possibly mediated by EMT, and suggest a mechanism for GS-incluced bronchial vasoconstriction.

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Documento generato il 15/07/20 alle ore 04:26:36