Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Maternal or fetal origin of rhesus monkey (Macaca mulatta) amniotic fluid leukocytes can be identified by polymerase chain reaction using the zinc finger Y gene
Autore:
Macias, AE; Wong, SW; Sadowsky, DW; Luetjens, CM; Axthelm, MK; Gravett, MG; Haluska, GJ; Novy, MJ;
Indirizzi:
Oregon Reg Primate Res Ctr, Div Reprod Sci, Beaverton, OR 97006 USA OregonReg Primate Res Ctr Beaverton OR USA 97006 Beaverton, OR 97006 USA Univ Guanajuato, Sch Med, Dept Microbiol, Leon, Mexico Univ Guanajuato Leon Mexico uato, Sch Med, Dept Microbiol, Leon, Mexico Oregon Reg Primate Res Ctr, Div Pathobiol & Immunol, Beaverton, OR 97006 USA Oregon Reg Primate Res Ctr Beaverton OR USA 97006 Beaverton, OR 97006 USA Univ Munster, Inst Reprod Med, D-4400 Munster, Germany Univ Munster Munster Germany D-4400 Reprod Med, D-4400 Munster, Germany Oregon Hlth Sci Univ, Dept Obstet & Gynecol, Portland, OR 97201 USA OregonHlth Sci Univ Portland OR USA 97201 ynecol, Portland, OR 97201 USA
Titolo Testata:
AMERICAN JOURNAL OF PRIMATOLOGY
fascicolo: 3, volume: 55, anno: 2001,
pagine: 159 - 170
SICI:
0275-2565(200111)55:3<159:MOFOOR>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
PRENATAL SEX DETERMINATION; DNA AMPLIFICATION; PERIPHERAL-BLOOD; CHROMOSOME; ZFY; SEQUENCE; CELLS; AMELOGENIN; EVOLUTION; PRIMATES;
Keywords:
Macaca mulatta; sex chromosomes; polymerase chain reaction; ZFY gene; amniotic fluid; leukocytes;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Novy, MJ Oregon Reg Primate Res Ctr, Div Reprod Sci, 505 NW 185th Ave, Beaverton, OR 97006 USA Oregon Reg Primate Res Ctr 505 NW 185th Ave Beaverton OR USA 97006
Citazione:
A.E. Macias et al., "Maternal or fetal origin of rhesus monkey (Macaca mulatta) amniotic fluid leukocytes can be identified by polymerase chain reaction using the zinc finger Y gene", AM J PRIMAT, 55(3), 2001, pp. 159-170

Abstract

Leukocytes can be found in substantial numbers within the intrauterine tissues and amniotic fluid of women, and play a central role in the pathophysiology of infection-related preterm labor by their production of proinflammatory mediators. It remains unclear whether these leukocytes represent a fetal immune response, a maternal response, or a combination of the two. The objective of this study was to develop a test in the rhesus monkey (Macaca mulatta) suitable for determining the percentage of male fetal cells presentin a population of leukocytes recovered from blood or amniotic fluid. We found inadequate specificity for rhesus monkey cells using commercial human Y-chromosome paint kits (fluorescence in situ hybridization (FISH)) Human-specific primers for the repetitive Y chromosome DYZ-1 locus employed in thepolymerase chain reaction (PCR) produced an unacceptable percentage of false positives. However, we successfully developed a PCR-based test using rhesus-specific primers for the zinc finger Y (ZFY) locus. Densitometry of PCRproducts from known ratios of male and female adult peripheral leukocytes generated a linear standard curve which provided quantitative results and required only 400 cells per sample. The rhesus beta globin (RBG) gene servedas an internal control. The PCR test correctly discriminated the sex of peripheral leukocytes in 20 adult males, 20 adult females, two male fetuses, and one female fetus. Serial samples of amniotic fluid from four chronically catheterized rhesus monkeys bearing male fetuses were used to confirm theutility of this assay for quantifying fetal cells in amniotic fluid. In conclusion, we have developed a PCR test which is suitable for distinguishingmale from female cells in adult and fetal blood and in amniotic fluid, which lends itself to a variety of diagnostic and biologic applications in therhesus monkey and potentially in other nonhuman primates. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 16:14:37