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Titolo:
cGMP abolishes agonist-induced [Ca2+](i) oscillations in human bladder epithelial cells
Autore:
Kwan, HY; Huang, Y; Kong, SK; Yao, X;
Indirizzi:
Chinese Univ Hong Kong, Fac Med, Dept Physiol, Hong Kong, Hong Kong, Peoples R China Chinese Univ Hong Kong Hong Kong Hong Kong Peoples R China oples R China Chinese Univ Hong Kong, Fac Med, Dept Biochem, Hong Kong, Hong Kong, Peoples R China Chinese Univ Hong Kong Hong Kong Hong Kong Peoples R China oples R China
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
fascicolo: 6, volume: 281, anno: 2001,
pagine: F1067 - F1074
SICI:
0363-6127(200112)281:6<F1067:CAA[OI>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
INOSITOL 1,4,5-TRISPHOSPHATE RECEPTOR; VASCULAR ENDOTHELIAL-CELLS; NITRIC-OXIDE SYNTHASE; CALCIUM OSCILLATIONS; INTRACELLULAR CALCIUM; RAT MEGAKARYOCYTES; URINARY-BLADDER; PROTEIN-KINASE; SMOOTH-MUSCLE; CA2+;
Keywords:
protein kinase G; inositol 1,4,5-triphosphate; calcium release; nitric oxide; intracellular calcium concentration; guanosine 3 ',5 '-cyclic monophosphate;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Yao, X Chinese Univ Hong Kong, Fac Med, Dept Physiol, Hong Kong, Hong Kong, Peoples R China Chinese Univ Hong Kong Hong Kong Hong Kong Peoples R China China
Citazione:
H.Y. Kwan et al., "cGMP abolishes agonist-induced [Ca2+](i) oscillations in human bladder epithelial cells", AM J P-REN, 281(6), 2001, pp. F1067-F1074

Abstract

Cytosolic calcium oscillations may permit cells to respond to information provided by increases in intracellular Ca2+ concentration ([Ca2+](i)) whileavoiding prolonged exposure to constantly elevated [Ca2+](i). In this study, we demonstrated that agonists could induce Ca2+ oscillations in human bladder epithelial cells. Application of 10 muM acetylcholine or 200 nM bradykinin triggered an initial Ca2+ transient that was followed by periodic [Ca2+](i) oscillations. The oscillations did not depend on extracellular Ca2+.8-Bromoguanosine 3', 5'-cyclic monophosphate abolished acetylcholine- or bradykinin-induced oscillations. Elevation of cellular cGMP by dipyridamole,an inhibitor of cGMP-specific phosphodiesterase, also terminated the [Ca2+](i) oscillations. The inhibitory effect of cGMP could be reversed by KT-5823, a highly specific inhibitor of protein kinase G (PKG), suggesting that the action of cGMP was mediated by PKG. Comparison of the effect of cGMP with that of xestospongin C, an inhibitor of the inositol 1,4,5-trisphosphate(IP3) receptor, revealed similarities between the action of cGMP and xestospongin C. Therefore, it is likely that cGMP and PKG may target a signal transduction step(s) linked to IP3 receptor-mediated Ca2+ release.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/10/20 alle ore 16:22:57