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Titolo:
RT-PCR reveals muscarinic acetylcholine receptor mRNA in the pre-Botzingercomplex
Autore:
Lai, JN; Shao, XM; Pan, RW; Dy, E; Huang, CH; Feldman, JL;
Indirizzi:
Univ Calif Los Angeles, Dept Neurobiol, Los Angeles, CA 90095 USA Univ Calif Los Angeles Los Angeles CA USA 90095 Los Angeles, CA 90095 USA
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
fascicolo: 6, volume: 281, anno: 2001,
pagine: L1420 - L1424
SICI:
1040-0605(200112)281:6<L1420:RRMARM>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
ROSTRAL VENTROLATERAL MEDULLA; PREBOTZINGER COMPLEX; RESPIRATORY RHYTHM; MESSENGER-RNAS; RAT; NEURONS; SUBTYPES; EXPRESSION; FAMILY; GENES;
Keywords:
reverse transcription-polymerase chain reaction; neural control of respiration; medullary slice; neonatal rat; messenger ribonucleic acid;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Feldman, JL Univ Calif Los Angeles, Dept Neurobiol, Box 951763, Los Angeles, CA 90095 USA Univ Calif Los Angeles Box 951763 Los Angeles CA USA 90095 USA
Citazione:
J.N. Lai et al., "RT-PCR reveals muscarinic acetylcholine receptor mRNA in the pre-Botzingercomplex", AM J P-LUNG, 281(6), 2001, pp. L1420-L1424

Abstract

Muscarinic receptors mediate the postsynaptic excitatory effects of acetylcholine (ACh) on inspiratory neurons in the pre-Botzinger complex (pre-BotC), the hypothesized site for respiratory rhythm generation. Because pharmacological tools for identifying the subtypes of the muscarinic receptors that underlie these effects are limited, we probed for mRNA for these receptors in the pre-BotC. We used RT-PCR to determine the expression of muscarinicreceptor subtypes in tissue punches of the pre-BotC taken from rat medullary slices. Cholinergic receptor subtype M-2 and M-3 mRNAs were observed in the first round of PCR amplification. All five subtypes, M-1-M-5, were observed in the second round of amplification. Our results suggest that the majority of muscarinic receptor subtypes in the pre-BotC are M-2 and M-3, withminor expression of M-1, M-4, and M-5.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/01/20 alle ore 03:11:49