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Titolo:
c-Myb influences HIV type 1 gene expression and virus production
Autore:
Churchill, MJ; Ramsay, RG; Rhodes, DI; Deacon, NJ;
Indirizzi:
Macfarlane Burnet Ctr Med Res, Natl Ctr HIV Virol Res, AIDS Mol Biol Unit,Fairfield, Vic 3078, Australia Macfarlane Burnet Ctr Med Res Fairfield VicAustralia 3078 078, Australia Peter MacCallum Canc Inst, Melbourne, Vic 3002, Australia Peter MacCallum Canc Inst Melbourne Vic Australia 3002 ic 3002, Australia
Titolo Testata:
AIDS RESEARCH AND HUMAN RETROVIRUSES
fascicolo: 16, volume: 17, anno: 2001,
pagine: 1481 - 1488
SICI:
0889-2229(200111)17:16<1481:CIHT1G>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-IMMUNODEFICIENCY-VIRUS; NORMAL HUMAN-LYMPHOCYTES; LONG TERMINAL REPEAT; DNA-BINDING ACTIVITY; HTLV-I; NUCLEOTIDE-SEQUENCE; T-CELLS; ACTIVATION; PROTEIN; PROTOONCOGENE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Churchill, MJ Macfarlane Burnet Ctr Med Res, Natl Ctr HIV Virol Res, AIDS Mol Biol Unit,Yarra Bend Rd, Fairfield, Vic 3078, Australia Macfarlane Burnet Ctr Med Res Yarra Bend Rd Fairfield Vic Australia 3078
Citazione:
M.J. Churchill et al., "c-Myb influences HIV type 1 gene expression and virus production", AIDS RES H, 17(16), 2001, pp. 1481-1488

Abstract

c-Myb is expressed in proliferating T cells. Fifteen c-Myb-binding sites can be identified in the HIV-1 long terminal repeat (LTR), suggesting that c-Myb may regulate HIV-1 gene expression and virus replication. Increasing the cellular levels of c-Myb by transient transfection of CEM cells resultedin a 10- to 20-fold activation of HIV-1 LTR-driven gene expression and mutation of one high-affinity Myb-binding site within the LTR reduced this activation by 60 to 70%. Conversely, inhibition of c-Myb expression in MT-2 cells by treatment with c-myb antisense oligonucleotides decreased HIV-1 replication by 85%, as measured by reverse transcriptase activity and cytopathic effects. The effect of c-myb antisense oligonucleotides on HIV-1 gene expression and virus particle production appeared to be independent of cell proliferation, but dependent on the presence of c-Myb activity mediated through the HIV-1 LTR. These data show that c-myb expression affects HIV-1 replication in CD4(+) T cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 20:08:59