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Titolo:
Diminished stimulation of glycosaminoglycan synthesis of human skin fibroblasts by interleukin-6 during in vitro aging
Autore:
Biecker, E; Schachtschabel, DO;
Indirizzi:
Univ Marburg, Inst Physiol Chem, D-35033 Marburg, Germany Univ Marburg Marburg Germany D-35033 siol Chem, D-35033 Marburg, Germany
Titolo Testata:
ZEITSCHRIFT FUR GERONTOLOGIE UND GERIATRIE
fascicolo: 5, volume: 34, anno: 2001,
pagine: 408 - 414
SICI:
0948-6704(200110)34:5<408:DSOGSO>2.0.ZU;2-R
Fonte:
ISI
Lingua:
GER
Soggetto:
HUMAN-DIPLOID FIBROBLASTS; NF-KAPPA-B; SYNOVIAL FIBROBLASTS; GROWTH-FACTORS; GENE-EXPRESSION; IN-VITRO; CELLS; WI-38; RESPONSIVENESS; ACTIVATION;
Keywords:
interleukin-6; fibroblasts; aging in vitro; glycosaminoglycans; hyaluronic acid;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Social & Behavioral Sciences
Clinical Medicine
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Schachtschabel, DO Wann 25C, D-35037 Marburg, Germany Wann 25C Marburg Germany D-35037 037 Marburg, Germany
Citazione:
E. Biecker e D.O. Schachtschabel, "Diminished stimulation of glycosaminoglycan synthesis of human skin fibroblasts by interleukin-6 during in vitro aging", Z GERON GER, 34(5), 2001, pp. 408-414

Abstract

Addition of human recombinant interleukin 6 (IL-6) to culture medium (supplemented MEM without or with 10% fetal calf serum (FCS)) of human skin fibroblasts exerted a stimulating effect in a dose-dependent manner on glycosaminoglycan (GAG) synthesis, including hyaluronic acid (hyaluronan) synthesis, of young (phase-II) skin fibroblasts in concentrations of 1 ng/ml and 10 ng/ml. Stimulation was mainly due to an increase in extracellular GAGs (secreted into culture medium), and to a lesser extent to an increase in peri- and intracellular GAGs. Stimulation with 1 ng/ml and 10 ng/ml IL-6 led to an increase in hyaluronic acid from 48% to 61% (-FCS) and from 77% to 90% (+10% FCS), respectively. Maximum stimulation, with and without FCS, was achived by 10 ng/ml IL-6. Compared to young (phase-II) cells, senescent (phase-III) cells, showed no significant stimulation of total GAG (including hyaluronic acid) synthesis by IL-6. The diminished response of GAG- and hyaluronic acid synthesis during agingof these in vitro cultured fibroblasts should motivate further research ifsimilar processes occur during aging in an organism.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/07/20 alle ore 16:07:14