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Titolo:
A recombinant virus assay using full-length envelope sequences to detect changes in HIV-1 co-receptor usage
Autore:
Dittmar, MT; Eichler, S; Reinberger, S; Henning, L; Krausslich, HG;
Indirizzi:
Univ Heidelberg, Inst Hyg, Abt Virol, D-69120 Heidelberg, Germany Univ Heidelberg Heidelberg Germany D-69120 , D-69120 Heidelberg, Germany Heinrich Pette Inst Expt Virol & Immunol, Abt Virol & Zellbiol, D-20251 Hamburg, Germany Heinrich Pette Inst Expt Virol & Immunol Hamburg Germany D-20251 Germany
Titolo Testata:
VIRUS GENES
fascicolo: 3, volume: 23, anno: 2001,
pagine: 281 - 290
SICI:
0920-8569(2001)23:3<281:ARVAUF>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
SIMIAN IMMUNODEFICIENCY VIRUS; CHEMOKINE RECEPTORS; DRUG SUSCEPTIBILITY; PROTEASE INHIBITORS; CORECEPTOR USAGE; TYPE-1 HIV-1; COILED-COIL; RESISTANCE; ENTRY; TROPISM;
Keywords:
recombinant virus assay; viral phenotype; resistance;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
50
Recensione:
Indirizzi per estratti:
Indirizzo: Dittmar, MT Univ Heidelberg, Inst Hyg, Abt Virol, Neuenheimer Feld 324, D-69120 Heidelberg, Germany Univ Heidelberg Neuenheimer Feld 324 Heidelberg Germany D-69120
Citazione:
M.T. Dittmar et al., "A recombinant virus assay using full-length envelope sequences to detect changes in HIV-1 co-receptor usage", VIRUS GENES, 23(3), 2001, pp. 281-290

Abstract

The clinical management of HIV-1 infection has benefited enormously from molecular characterization of drug resistance as well as determination of the viral phenotype in vitro. HIV-1 infected individuals on HAART are currently monitored for the development of drug resistance variants allowing clinicians to redesign drug regimens. An understanding of the molecular basis ofthe evolution of drug resistance in vivo allows the improvement of the drugs as well as in vitro evaluation of new antiviral compounds alone or in combination with those currently approved. New findings suggest that viral envelopes could be a target to inhibit infection and replication. Therefore the generation of a recombinant virus assay (RVA) to allow the phenotypic determination of drug resistance against entry inhibitors (EI) is anticipated. We constructed an env-deleted clone of HIV-1 using the molecular clone NL-4.3. PCR amplified complete envelope genes (NL-4.3, BaL, primary envelope-genes) were ligated in vitro with a deletion clone (pNL-DeltaK) and PM1-cells, supporting the replication of R5- and X4-tropic viruses, were transfected. Determination of co-receptor usage of the harvested recombinant virus-swarm revealed no difference compared to the molecular clones derived individually from three different patients. These results clearly show that an envelope-based RVA is practicable to monitor HIV-co-receptor usage at a giventime point. Furthermore, this assay will allow to monitor resistance development against existing and future entry inhibitors and will aid to improvethe management of HIV-therapy.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 15:46:09