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Titolo:
Drosophila amphiphysin is a post-synaptic protein required for normal locomotion but not endocytosis
Autore:
Leventis, PA; Chow, BM; Stewart, BA; Iyengar, B; Campos, AR; Boulianne, GL;
Indirizzi:
Univ Toronto, Dept Zool, Toronto, ON, Canada Univ Toronto Toronto ON Canada v Toronto, Dept Zool, Toronto, ON, Canada Univ Toronto, Dept Mol & Med Genet, Toronto, ON, Canada Univ Toronto Toronto ON Canada Dept Mol & Med Genet, Toronto, ON, Canada Hosp Sick Children, Program Dev Biol, Toronto, ON M5G 1X8, Canada Hosp Sick Children Toronto ON Canada M5G 1X8 Toronto, ON M5G 1X8, Canada McMaster Univ, Dept Biol, Hamilton, ON L8S 4K1, Canada McMaster Univ Hamilton ON Canada L8S 4K1 ol, Hamilton, ON L8S 4K1, Canada
Titolo Testata:
TRAFFIC
fascicolo: 11, volume: 2, anno: 2001,
pagine: 839 - 850
SICI:
1398-9219(200111)2:11<839:DAIAPP>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
RECEPTOR-MEDIATED ENDOCYTOSIS; VESICLE ENDOCYTOSIS; TUMOR-SUPPRESSOR; PLASMA-MEMBRANE; SH3 DOMAIN; GENE; DYNAMIN; BINDING; VIABILITY; CLATHRIN;
Keywords:
actin; amphiphysin; endocytosis; synaptic vesicle recycling;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Boulianne, GL Univ Toronto, Dept Zool, 25 Harbord St, Toronto, ON, Canada Univ Toronto 25 Harbord St Toronto ON Canada to, ON, Canada
Citazione:
P.A. Leventis et al., "Drosophila amphiphysin is a post-synaptic protein required for normal locomotion but not endocytosis", TRAFFIC, 2(11), 2001, pp. 839-850

Abstract

Clathrin-mediated endocytosis is required to recycle synaptic vesicles forfast and efficient neurotransmission. Amphiphysins are thought to be multiprotein adaptors that may contribute to this process by bringing together many of the proteins required for endocytosis. Their in vivo function, however, has yet to be determined. Here, we show that the Drosophila genome encodes a single amphiphysin gene that is broadly expressed during development. We also show that, unlike its vertebrate counterparts, Drosophila Amphiphysin is enriched postsynaptically at the larval neuromuscular junction. To determine the role of Drosophila Amphiphysin, we also generated null mutantswhich are viable but give rise to larvae and adults with pronounced locomotory defects. Surprisingly, the locomotory defects cannot be accounted for by alterations in the morphology or physiology of the neuromuscular junction. Moreover, using stimulus protocols designed to test endocytosis under moderate and extreme vesicle cycling, we could not detect any defect in the neuromuscular junction of the amphiphysin mutant. Taken together, our findings suggest that Amphiphysin is not required for viability, nor is it absolutely required for clathrin-mediated endocytosis. However, Drosophila Amphiphysin function is required in both larvae and adults for normal locomotion.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/07/20 alle ore 23:01:23