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Titolo:
Probing the machinery of intracellular trafficking with the atomic force microscope
Autore:
Kumar, S; Hoh, JH;
Indirizzi:
Johns Hopkins Univ, Sch Med, Dept Physiol, Baltimore, MD 21205 USA Johns Hopkins Univ Baltimore MD USA 21205 hysiol, Baltimore, MD 21205 USA Johns Hopkins Univ, Dept Chem Engn, Baltimore, MD 21218 USA Johns Hopkins Univ Baltimore MD USA 21218 m Engn, Baltimore, MD 21218 USA
Titolo Testata:
TRAFFIC
fascicolo: 11, volume: 2, anno: 2001,
pagine: 746 - 756
SICI:
1398-9219(200111)2:11<746:PTMOIT>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
DIRECT VISUALIZATION; SYNAPTIC VESICLES; ADHESION FORCES; CELLS; MICROTUBULES; DYNAMICS; ACTIN; AFM; SPECTROSCOPY; CYTOSKELETON;
Keywords:
atomic force microscopy; AFM; cell mechanics; cytoskeleton; force measurements; imaging; membrane fusion; receptors; vesicles;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
63
Recensione:
Indirizzi per estratti:
Indirizzo: Hoh, JH Johns Hopkins Univ, Sch Med, Dept Physiol, Baltimore, MD 21205 USAJohns Hopkins Univ Baltimore MD USA 21205 Baltimore, MD 21205 USA
Citazione:
S. Kumar e J.H. Hoh, "Probing the machinery of intracellular trafficking with the atomic force microscope", TRAFFIC, 2(11), 2001, pp. 746-756

Abstract

Atomic force microscopy has emerged as a powerful tool for characterizing single biological macromolecules, macromolecular assemblies, and whole cells in aqueous buffer, in real time, and at molecular-scale spatial and forceresolution. Many of the central elements of intracellular transport are tens to hundreds of nanometers in size and highly dynamic. Thus, atomic forcemicroscopy provides a valuable means of addressing questions of structure and mechanism in intracellular transport. We begin this review of recent efforts to apply atomic force microscopy to problems in intracellular transport by discussing the technical principles behind atomic force microscopy. We then turn to three specific areas in which atomic force microscopy has been applied to problems with direct implications for intracellular trafficking: cytoskeletal structure and dynamics, vesicular transport, and receptor-ligand interactions. In each case, we discuss studies which use both intactcellular elements and reconstituted models. While many technical challenges remain, these studies point to several areas where atomic force microscopy can be used to provide valuable insight into intracellular transport at exquisite spatial and energetic resolution.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/01/20 alle ore 07:10:34