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Titolo:
A novel use of alginate hydrogel as Schwann cell matrix
Autore:
Mosahebi, A; Simon, M; Wiberg, M; Terenghi, G;
Indirizzi:
Royal Free & Univ Coll Med Sch, Univ Dept Surg, Blond McIndoe Ctr, London NW3 2PF, England Royal Free & Univ Coll Med Sch London England NW3 2PF n NW3 2PF, England Umea Univ, Dept Hand & Plast Surg & Anat, Umea, Sweden Umea Univ Umea Sweden Univ, Dept Hand & Plast Surg & Anat, Umea, Sweden
Titolo Testata:
TISSUE ENGINEERING
fascicolo: 5, volume: 7, anno: 2001,
pagine: 525 - 534
SICI:
1076-3279(200110)7:5<525:ANUOAH>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERIPHERAL-NERVE REGENERATION; RAT SCIATIC-NERVE; FIBRIN GLUE; EXTRACELLULAR-MATRIX; HYALURONIC-ACID; IN-VIVO; NEUROTROPHIC FACTOR; REPAIR; TRANSPLANTATION; CARTILAGE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Terenghi, G Royal Free & Univ Coll Med Sch, Univ Dept Surg, Blond McIndoe Ctr, Royal Free Campus,Rowland Hill St, London NW3 2PF, England Royal Free & Univ Coll Med Sch Royal Free Campus,Rowland Hill St London England NW3 2PF
Citazione:
A. Mosahebi et al., "A novel use of alginate hydrogel as Schwann cell matrix", TISSUE ENG, 7(5), 2001, pp. 525-534

Abstract

The use of bioresorbable conduits supplemented with Schwann cells (SCs) isa promising tissue engineering technique to replace nerve grafting. Alginate hydrogel (AH), as a SC tissue engineering matrix, has many advantages over previously used matrices but has not been evaluated for this purpose. Inthis study, the viability and proliferation of SCs together with SC function in AH was evaluated in vitro. AlamarBlue(TM) cell assay was used to monitor the viability of SCs in AH and compared to SC viability in collagen gel, fibrin glue, hyaluronic acid, Matrigel(R), and standard culture plate over 5 days in culture. The results showed that the viability and growth of SCs in different matrices over the culture period did not significantly differ to culture plate culture. SC function when suspended in AH was monitored using chick embryo dorsal root ganglia (CDRG) growth assay. Growth of CDRG in AH with or without SCs was compared to CDRG growth without AH matrix. After 3 days in culture, the mean length of neurite sprouting was measured. The results showed that there was neurite growth in AH but was reduced to 43% of control. The neurite growth in AH was, however, enhanced by 170% when SCs were suspended in the gel. In conclusion, AH supported SC viability andfunction in vitro and may be useful in peripheral nerve tissue engineeringin reconstructive procedures.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 05:08:37