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Titolo:
Molecular detection and characterization of circulating tumor cells and micrometastases in prostatic, urothelial, and renal cell carcinomas
Autore:
Ghossein, RA; Bhattacharya, S;
Indirizzi:
Mem Sloan Kettering Canc Ctr, Dept Pathol, New York, NY 10021 USA Mem Sloan Kettering Canc Ctr New York NY USA 10021 New York, NY 10021 USA
Titolo Testata:
SEMINARS IN SURGICAL ONCOLOGY
fascicolo: 4, volume: 20, anno: 2001,
pagine: 304 - 311
SICI:
8756-0437(200106)20:4<304:MDACOC>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE-CHAIN-REACTION; MEMBRANE ANTIGEN PSMA; REVERSE-TRANSCRIPTASE; PERIPHERAL-BLOOD; CANCER-CELLS; RADICAL PROSTATECTOMY; BONE-MARROW; BIOCHEMICAL RECURRENCE; LYMPH-NODES; RT-PCR;
Keywords:
minimal residual disease; reverse transcriptase; polymerase chain reaction; molecular diagnostics; prostatic carcinoma; urothelial carcinoma; renal cell carcinoma;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Ghossein, RA Mem Sloan Kettering Canc Ctr, Dept Pathol, 1275 York Ave, NewYork, NY 10021 USA Mem Sloan Kettering Canc Ctr 1275 York Ave New York NY USA 10021
Citazione:
R.A. Ghossein e S. Bhattacharya, "Molecular detection and characterization of circulating tumor cells and micrometastases in prostatic, urothelial, and renal cell carcinomas", SEM SURG ON, 20(4), 2001, pp. 304-311

Abstract

The detection and molecular characterization of circulating tumor cells (CTCs) and micrometastases in urinary tract and prostatic tumors may have important prognostic and therapeutic implications. In the last decade, numerous groups have attempted the detection of occult tumor cells in renal, prostatic, and urothelial carcinomas using the highly sensitive reverse-transcriptase polymerase chain reaction (RT-PCR). In prostatic carcinoma (PC), tissue-specific transcripts were detected with high specificity in the blood ofpatients with localized and advanced disease. PCR assays for PC detection were shown to be strong predictors of poorer outcome in some reports, whilea lack of prognostic significance was found in other studies. There was a vast difference in the PCR positivity rates between various groups studyingPC. This discrepancy could be due to variations in PCR methodology. In urothelial and renal cell carcinoma, the amount of research on the subject is still too limited. Currently, these assays for occult tumor cells are promising but are not yet ready to use in PC and urinary tract tumors. Because of the many limitations of PCR (e.g., false positives), many groups are developing new approaches for the detection of occult tumor cells. The most attractive technique involves immunomagnetic isolation of intact CTC and micrometastases prior to downstream analysis. The tumor-rich magnetic fraction can be subjected to RT-PCR, immunocytochemistry, and in situ hybridization. This will lead to better quantification and molecular characterization of these tumor cells. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 09:35:58