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Titolo:
Differential expression and characterization analysis of a new gene with WD domains in fish oogenesis
Autore:
Wen, JJ; Xie, J; Liu, SG; Gui, JF;
Indirizzi:
Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China Chinese Acad Sci Wuhan Peoples R China 430072 an 430072, Peoples R China Sichuan Univ, Coll Life Sci, Chengdu 610064, Peoples R China Sichuan UnivChengdu Peoples R China 610064 ngdu 610064, Peoples R China
Titolo Testata:
SCIENCE IN CHINA SERIES C-LIFE SCIENCES
fascicolo: 5, volume: 44, anno: 2001,
pagine: 541 - 553
SICI:
1006-9305(200110)44:5<541:DEACAO>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
SILVER CRUCIAN CARP; GROWTH-FACTOR-BETA; TROUT ONCORHYNCHUS-MYKISS; XENOPUS-LAEVIS OOCYTES; AURATUS GIBELIO BLOCH; I IGF-I; RAINBOW-TROUT; INVOLVEMENT; SUPPRESSION; RECEPTORS;
Keywords:
silver crucian carp; oocyte; oogenesis; suppressive subtractive hybridization; WD domain;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Gui, JF Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China Chinese Acad Sci Wuhan Peoples R China 430072 2, Peoples R China
Citazione:
J.J. Wen et al., "Differential expression and characterization analysis of a new gene with WD domains in fish oogenesis", SCI CHINA C, 44(5), 2001, pp. 541-553

Abstract

A new gene with WD domains is cloned and characterized according to its differential transcription and expression between previtellogenic oocytes (phase I oocytes) and fully-grown oocytes (phase V oocytes) from natural gynogenetic silver crucian carp (Carassius auratus gibelio) by using the combinative methods of suppressive subtraction hybridization, SMART cDNA synthesisand RACE-PCR. The full-length cDNA is 1870 bp. Its 5 ' untranslated regionis 210 bp, followed by an open reading frame of 990 bp, which has the typical vertebrate initiator codon of ANNATG. The open reading frame encodes a protein with 329 amino acids. It has 670 bp of 3 ' untranslated region and an AATAAA polyadenylation signal. Because it has 92% homology to STRAP (serine-threonine kinase receptor-associated protein), a recently reported gene, we named it FSTRAP (fish STRAP). Virtual Northern blotting indicated thatthe FSTRAP was transcribed in fully-grown oocytes (phase V oocytes), but not in previtellogenic oocytes (phase I oocytes). RT-PCR analysis showed that FSTRAP was transcribed in brain, heart, kidney, muscle, ovary, spleen andtestis, but not in liver. And its mRNA could be detected in the oocytes from phase II to phase V. Western blotting also showed that FSTRAP protein could be detected in brain, heart, kidney, muscle, ovary, spleen and testis except liver. Results of Western blotting on various oocytes were also similar to the RT-PCR data. FSTRAP protein was not expressed in the previtellogenic oocytes. Its expression initiated from phase II oocytes after vitellogenesis, and was consistent with the mRNA transcription.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 15:07:08