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Titolo:
Screening for strongly regenerative genotypes of spinach in tissue cultureusing subcultured root explants
Autore:
Ishizaki, T; Komai, F; Masuda, K;
Indirizzi:
Hokkaido Univ, Grad Sch Agr, Sapporo, Hokkaido 0608589, Japan Hokkaido Univ Sapporo Hokkaido Japan 0608589 oro, Hokkaido 0608589, Japan
Titolo Testata:
PLANT CELL TISSUE AND ORGAN CULTURE
fascicolo: 3, volume: 67, anno: 2001,
pagine: 251 - 255
SICI:
0167-6857(200112)67:3<251:SFSRGO>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
SOMATIC EMBRYOGENESIS; PLANT-REGENERATION; OLERACEA L; ALFALFA; SEGMENTS; CALLUS; CULTIVARS;
Keywords:
callus; gibberellic acid; root culture; somatic embryogenesis; Spinacia oleracea L.;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Ishizaki, T Hokkaido Univ, Grad Sch Agr, Sapporo, Hokkaido 0608589, Japan Hokkaido Univ Sapporo Hokkaido Japan 0608589 o 0608589, Japan
Citazione:
T. Ishizaki et al., "Screening for strongly regenerative genotypes of spinach in tissue cultureusing subcultured root explants", PL CELL TIS, 67(3), 2001, pp. 251-255

Abstract

A system for subculture of spinach (Spinacia oleracea L.) roots was established, and differences in regeneration; namely, embryogenic competence, among individuals of the 'Nippon' cultivar were examined. Root tissues, excised from seedlings, were grown on medium without growth regulators and subcultured on the same medium and then on medium that contained 10 muM naphthaleneacetic acid and 0.1 muM gibberellic acid to induce callus formation. Calli were transferred to medium without growth regulators. All explants formedcalli. However, the frequency of embryo formation varied among lines. Higher concentrations of gibberellic acid in the callus-induction medium had limited effects on somatic embryogenesis from poorly embryogenic lines. Theseresults indicate that inherent factors are important for somatic embryogenesis in spinach and that the root subculture system is useful for identifying strongly regenerative genotypes among individuals of a single cultivar.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 12:36:27