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Titolo:
Scattered-light imaging in vivo tracks fast and slow processes of neurophysiological activation
Autore:
Rector, DM; Rogers, RF; Schwaber, JS; Harper, RM; George, JS;
Indirizzi:
Los Alamos Natl Lab, Biophys Grp, Los Alamos, NM 87545 USA Los Alamos NatlLab Los Alamos NM USA 87545 Grp, Los Alamos, NM 87545 USA Thomas Jefferson Univ, Dept Pathol Anat & Cell Biol, Philadelphia, PA 19107 USA Thomas Jefferson Univ Philadelphia PA USA 19107 hiladelphia, PA 19107 USA Univ Calif Los Angeles, Dept Neurobiol, Los Angeles, CA 90095 USA Univ Calif Los Angeles Los Angeles CA USA 90095 Los Angeles, CA 90095 USA
Titolo Testata:
NEUROIMAGE
fascicolo: 5, volume: 14, anno: 2001,
pagine: 977 - 994
SICI:
1053-8119(200111)14:5<977:SIIVTF>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
NUCLEUS-TRACTUS-SOLITARIUS; RAT BARREL CORTEX; INTRINSIC OPTICAL SIGNALS; FREELY BEHAVING ANIMALS; FUNCTIONAL ARCHITECTURE; NEURAL ACTIVITY; IN-VIVO; NONINVASIVE DETECTION; ELECTRICAL-ACTIVITY; NEURONAL-ACTIVITY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
56
Recensione:
Indirizzi per estratti:
Indirizzo: George, JS Los Alamos Natl Lab, Biophys Grp, MS-D454,P-21,POB 1663, Los Alamos, NM 87545 USA Los Alamos Natl Lab MS-D454,P-21,POB 1663 Los Alamos NM USA 87545
Citazione:
D.M. Rector et al., "Scattered-light imaging in vivo tracks fast and slow processes of neurophysiological activation", NEUROIMAGE, 14(5), 2001, pp. 977-994

Abstract

We imaged fast optical changes associated with evoked neural activation inthe dorsal brainstem of anesthetized rats, using a novel imaging device. The imager consisted of a gradient-index (GRIN) lens, a microscope objective, and a miniature charged-coupled device (CCD) video camera. We placed the probe in contact with tissue above cardiorespiratory areas of the nucleus of the solitary tract and illuminated the tissue with 780-nm light through flexible fibers around the probe perimeter. The focus depth was adjusted by moving the camera and microscope objective relative to the fixed GRIN lens. Back-scattered light images were relayed through the GRIN lens to the CCD camera. Video frames were digitized at 100 frames per second, along with tracheal pressure, arterial blood pressure, and electrocardiogram signals recorded at 1 kHz per channel. A macroelectrode placed under the GRIN lens recorded field potentials from the imaged area. Aortic, vagal, and superior laryngeal nerves were dissected free of surrounding tissue within the neck. Separate shocks to each dissected nerve elicited evoked electrical responsesand caused localized optical activity patterns. The optical response was modeled by four distinct temporal components corresponding to putative physical mechanisms underlying scattered light changes. Region-of-interest analysis revealed image areas which were dominated by one or more of the different time-course components, some of which were also optimally recorded at different tissue depths. Two slow optical components appear to correspond to hemodynamic responses to metabolic demand associated with activation. Two fast optical components paralleled electrical evoked responses. (C) 2001 Academic Press.

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Documento generato il 18/01/20 alle ore 02:33:52