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Titolo:
Biopanning and rapid analysis of selective interactive ligands
Autore:
Giordano, RJ; Cardo-Vila, M; Lahdenranta, J; Pasqualini, R; Arap, W;
Indirizzi:
Univ Texas, MD Anderson Canc Ctr, Houston, TX 77030 USA Univ Texas Houston TX USA 77030 Anderson Canc Ctr, Houston, TX 77030 USA
Titolo Testata:
NATURE MEDICINE
fascicolo: 11, volume: 7, anno: 2001,
pagine: 1249 - 1253
SICI:
1078-8956(200111)7:11<1249:BARAOS>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENDOTHELIAL GROWTH-FACTOR; TRYPANOSOMA-CRUZI; PEPTIDES; BINDING; LIBRARIES; NEUROPILIN-1; RECEPTORS; PHAGE; CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
16
Recensione:
Indirizzi per estratti:
Indirizzo: Pasqualini, R Univ Texas, MD Anderson Canc Ctr, 1515 Holcombe Blvd, Houston, TX 77030 USA Univ Texas 1515 Holcombe Blvd Houston TX USA 77030 77030 USA
Citazione:
R.J. Giordano et al., "Biopanning and rapid analysis of selective interactive ligands", NAT MED, 7(11), 2001, pp. 1249-1253

Abstract

Here we introduce a new approach for the screening, selection and sorting of cell-surface-binding peptides from phage libraries. Biopanning and rapidanalysis of selective interactive ligands (termed BRASIL) is based on differential centrifugation in which a cell suspension incubated with phage in an aqueous upper phase is centrifuged through a non-miscible organic lower phase. This single-step organic phase separation is faster, more sensitive and more specific than current methods that rely on washing steps or limiting dilution. As a proof-of-principle, we screened human endothelial cells stimulated with vascular endothelial growth factor (VEGF) and constructed a peptide-based ligand-receptor map of the VEGF family. Next, we validated the motif PQPRPL as a novel chimeric ligand mimic that binds specifically to VEGF receptor-1 and to neuropilin-1. BRASIL may prove itself a superior method for probing target cell surfaces with a broad range of potential applications.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/07/20 alle ore 22:26:57