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Titolo:
MpI ligand increases P2Y1 receptor gene expression in megakaryocytes with no concomitant change in platelet response to ADP
Autore:
Hechler, BA; Toselli, P; Ravanat, C; Gachet, C; Ravid, K;
Indirizzi:
Boston Univ, Sch Med, Dept Biochem, Whitaker Cardiovasc Inst, Boston, MA 02118 USA Boston Univ Boston MA USA 02118 ker Cardiovasc Inst, Boston, MA 02118 USA INSERM U311, Etablissement Francais Sang Alsasce, Strasbourg, France INSERM U311 Strasbourg France Francais Sang Alsasce, Strasbourg, France
Titolo Testata:
MOLECULAR PHARMACOLOGY
fascicolo: 5, volume: 60, anno: 2001,
pagine: 1112 - 1120
SICI:
0026-895X(200111)60:5<1112:MLIPRG>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
P2Y(1) RECEPTOR; FUNCTIONAL-CHARACTERIZATION; NONHUMAN-PRIMATES; MOLECULAR-BASIS; AGGREGATION; ACTIVATION; THROMBOPOIETIN; GROWTH; CELLS; PURINOCEPTORS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Ravid, K Boston Univ, Sch Med, Dept Biochem, Whitaker Cardiovasc Inst, 715Albany St,K225, Boston, MA 02118 USA Boston Univ 715 Albany St,K225 BostonMA USA 02118 , MA 02118 USA
Citazione:
B.A. Hechler et al., "MpI ligand increases P2Y1 receptor gene expression in megakaryocytes with no concomitant change in platelet response to ADP", MOLEC PHARM, 60(5), 2001, pp. 1112-1120

Abstract

AV6The P2Y(1) receptor is responsible for the initiation of platelet aggregation in response to ADP and plays a key role in thrombosis. Although thisreceptor is expressed early in the platelet lineage, the regulation of itsexpression during megakaryocyte differentiation is unknown. In the mouse megakaryocytic cell line Y10/L8057, we detected P2Y(1) mRNA of three sizes (2.5, 4.4, and 7.4 kb). These cells have previously been shown to respond toMpl ligand, the pivotal regulator of megakaryocytopoiesis, by increasing their expression of differentiation markers. MpI ligand enhanced levels of P2Y(1) mRNAs in Y10/L8057 cells and this effect was selective: the same cytokine did not increase levels of A2a adenosine receptor mRNA. Although Mpl ligand did not affect the short half-lives of the P2Y(1) mRNAs, it enhanced transcription of the P2Y, gene. It also increased cell size and the number of cell surface P2Y(1) receptors, but not P2Y(1) receptor density. Injection of Mpl ligand into mice upregulated P2Y(1) receptor mRNAs in megakaryocytes, as shown by in situ hybridization. However, platelets isolated from these mice did not exhibit a higher P2Y(1) receptor density or increased reactivity to AIDP. This correlates with the finding that MpI ligand increases GPIIb mRNA in megakaryocytes but not the density of the protein per platelet. Thus, the enhancement of P2Y(1) receptor expression induced by MpI ligandin megakaryocytes may be an integral feature of their differentiation, whereas clinical use of this compound might not be associated with platelet hyper-reactivity to ADP.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 09:25:10