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Titolo:
Expression of a Y-box protein, YB2/RYB-a, precedes protamine 2 expression during spermatogenesis in rodents
Autore:
Iuchi, Y; Kobayashi, T; Kaneko, T; Takahara, M; Ogino, T; Fujii, J;
Indirizzi:
Yamagata Univ, Sch Med, Dept Biochem, Yamagata 9909585, Japan Yamagata Univ Yamagata Japan 9909585 pt Biochem, Yamagata 9909585, Japan Yamagata Univ, Sch Med, Dept Orthoped, Yamagata 9909585, Japan Yamagata Univ Yamagata Japan 9909585 t Orthoped, Yamagata 9909585, Japan
Titolo Testata:
MOLECULAR HUMAN REPRODUCTION
fascicolo: 11, volume: 7, anno: 2001,
pagine: 1023 - 1031
SICI:
1360-9947(200111)7:11<1023:EOAYPY>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACID-BINDING-PROTEINS; CYCLIN-DEPENDENT KINASE-5; MESSENGER-RNA; GERM-CELLS; COLD-SHOCK; TRANSLATIONAL REGULATION; TRANSCRIPTION FACTOR; HUMAN SPERMIOGENESIS; ESCHERICHIA-COLI; MAMMALIAN TESTIS;
Keywords:
in-situ hybridization; protamine; spermatogenesis; translational repression; Y-box proteins;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Fujii, J Yamagata Univ, Sch Med, Dept Biochem, 2-2-2 Iidanishi, Yamagata 9909585, Japan Yamagata Univ 2-2-2 Iidanishi Yamagata Japan 9909585 9585, Japan
Citazione:
Y. Iuchi et al., "Expression of a Y-box protein, YB2/RYB-a, precedes protamine 2 expression during spermatogenesis in rodents", MOL HUM REP, 7(11), 2001, pp. 1023-1031

Abstract

Y-box binding proteins, a large family of proteins, are involved in a variety of functions. The present study describes the expression of YB2, a rat Y-box binding protein, and/or RYB-a, an alternatively spliced product of the YB2 gene during spermatogenesis. YB2/RYB-a is thought to be the rat orthologue of mouse Y-box protein 3 (MSY3). An antibody which recognizes YB2/RYB-a was developed and applied in an immunochemical study of rat and mouse testes. We also carried out an in-situ hybridization study and Northern blot analysis of YB2/RYB-a and protamine 2 mRNA expression. Both YB2/RYB-a mRNA and the proteins appeared in prepubertal mouse testes, prior to the expression of the mouse protamine 2 mRNA. The mRNA and protein were present at high levels in spermatocytes, decreased in round to elongated spermatids, and were absent in spermatozoa. Since the protamine 2 mRNA was present at high levels in round and elongating spermatids, the proposed function of the YB2/RYB-a protein as a translational repressor of the mRNA was supported in mouse. The level and localization of YB2/RYB-a mRNA and protein expression inthe rat testis was comparable to that in mouse testis, although rat testisis known to express a very low level of protamine 2, but is also likely toaffect the expression of other proteins (including protamine 1) during spermatogenesis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/04/20 alle ore 03:00:08