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Titolo:
A modified mutagenic PCR-RFLP method for K-ras codon 12 and 13 mutations detection in NSCLC patients
Autore:
Hatzaki, A; Razi, E; Anagnostopoulou, K; Iliadis, K; Kodaxis, A; Papaioannou, D; Labropoulos, S; Vasilaki, M; Kosmidis, P; Saetta, A; Mihalatos, M; Nasioulas, G;
Indirizzi:
Diagnost & Therapeut Ctr Athens HYGEIA, Dept Mol Biol, Athens 15123, Greece Diagnost & Therapeut Ctr Athens HYGEIA Athens Greece 15123 15123, Greece Diagnost & Therapeut Ctr Athens HYGEIA, Oncol Clin 1, Athens 15123, GreeceDiagnost & Therapeut Ctr Athens HYGEIA Athens Greece 15123 15123, Greece Diagnost & Therapeut Ctr Athens HYGEIA, Thorac Surg Clin, Athens 15123, Greece Diagnost & Therapeut Ctr Athens HYGEIA Athens Greece 15123 15123, Greece Diagnost & Therapeut Ctr Athens HYGEIA, Dept Pathol Anat, Athens 15123, Greece Diagnost & Therapeut Ctr Athens HYGEIA Athens Greece 15123 15123, Greece Diagnost & Therapeut Ctr Athens HYGEIA, Oncol Clin, Athens 15123, Greece Diagnost & Therapeut Ctr Athens HYGEIA Athens Greece 15123 15123, Greece Diagnost & Therapeut Ctr Athens HYGEIA, Oncol Clin 2, Athens 15123, GreeceDiagnost & Therapeut Ctr Athens HYGEIA Athens Greece 15123 15123, Greece Univ Athens, Sch Med, Dept Pathol Anat, GR-11527 Athens, Greece Univ Athens Athens Greece GR-11527 Pathol Anat, GR-11527 Athens, Greece
Titolo Testata:
MOLECULAR AND CELLULAR PROBES
fascicolo: 5, volume: 15, anno: 2001,
pagine: 243 - 247
SICI:
0890-8508(200110)15:5<243:AMMPMF>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELL LUNG-CANCER; GENE-MUTATIONS; PROGNOSTIC MARKER; POINT MUTATIONS; STAGE-I; ONCOGENE; ADENOCARCINOMA; ACTIVATION; CARCINOMA; TUMORS;
Keywords:
K-ras; mutation detection; mutagenic PCR; NSCLC;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: Nasioulas, G Diagnost & Therapeut Ctr Athens HYGEIA, Dept Mol Biol, 4 Erythrou Stavrou & Kifissias Ave, Athens 15123, Greece Diagnost & Therapeut CtrAthens HYGEIA 4 Erythrou Stavrou & Kifissias Ave Athens Greece 15123
Citazione:
A. Hatzaki et al., "A modified mutagenic PCR-RFLP method for K-ras codon 12 and 13 mutations detection in NSCLC patients", MOL CELL PR, 15(5), 2001, pp. 243-247

Abstract

Evidence from many investigators has shown that mutations in the first exon of K-ras gene occur at elevated frequencies in lung, pancreatic and coloncarcinoma and seem to be of prognostic importance. The aim of this study was to develop an effective method for the detection of K-ras mutations in codons 12 and 13 in non-small-cell lung cancer (NSCLC) patients in order to investigate correlation with clinical outcome. DNA was extracted from tumour and neighbouring non-neoplastic lung tissuesfrom 70 patients and screened for codon 12 and 13 mutations. We applied a mutagenic PCR-restriction fragment length polymorphism for both codon 12 and 13 mutation detection. Codon 12 mutation was identified in 20% of NSCLC patients, whereas no codon 13 mutation was detected. As expected, the respective non-neoplastic tissues exhibited no mutations. We observed an increased codon 12 mutation prevalence in adenocarcinoma comparing to other types of carcinomas. Follow-up for 29 patients with a mean time of 12 months indicates an increased relapse rate in NSCLC patients with the K-ras codon 12 mutation. Furthermore, a trend towards increased percentage of mutant sampleswas observed in the advanced stage group of patients. We provide evidence that our approach is a fast and reliable method for screening K-ras exon 1 mutations in tumour samples from NSCLC patients. (C) 2001 Academic Press.

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Documento generato il 25/01/20 alle ore 18:34:39