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Titolo:
Identification and characterization of functional angiotensin II type I receptors on immortalized human fetal aortic vascular smooth muscle cells
Autore:
Martin, MM; Victor, X; Zhao, X; McDougall, JK; Elton, TS;
Indirizzi:
Brigham Young Univ, Dept Chem & Biochem, Provo, UT 84602 USA Brigham YoungUniv Provo UT USA 84602 Chem & Biochem, Provo, UT 84602 USA Univ Washington, Fred Hutchinson Canc Res Ctr, Seattle, WA 98195 USA Univ Washington Seattle WA USA 98195 Canc Res Ctr, Seattle, WA 98195 USA
Titolo Testata:
MOLECULAR AND CELLULAR ENDOCRINOLOGY
fascicolo: 1-2, volume: 183, anno: 2001,
pagine: 81 - 91
SICI:
0303-7207(20011025)183:1-2<81:IACOFA>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
SPONTANEOUSLY HYPERTENSIVE RATS; WISTAR-KYOTO RATS; PROTEIN-SYNTHESIS; GENOMIC ORGANIZATION; MOLECULAR-CLONING; GROWTH-RESPONSE; GENE-EXPRESSION; MESSENGER-RNAS; BLOOD-PRESSURE; UP-REGULATION;
Keywords:
angiotensin II receptors; signal transduction; gene regulation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
61
Recensione:
Indirizzi per estratti:
Indirizzo: Elton, TS Brigham Young Univ, Dept Chem & Biochem, C206 Benson Sci Bldg,POB 25700, Provo, UT 84602 USA Brigham Young Univ C206 Benson Sci Bldg,POB 25700 Provo UT USA 84602
Citazione:
M.M. Martin et al., "Identification and characterization of functional angiotensin II type I receptors on immortalized human fetal aortic vascular smooth muscle cells", MOL C ENDOC, 183(1-2), 2001, pp. 81-91

Abstract

Studies investigating the mechanisms that govern the expression of the human angiotensin II type 1 receptor (hAT(1)R) gene have progressed slowly dueto the lack of human cell lines that express the AT(1)R. Recently, however, an immortalized human fetal aortic vascular smooth muscle cell line (FLTR) was generated using an amphotropic recombinant retroviral construct containing the E6/E7 open reading frames of the human papillomavirus type 16. Radioligand binding studies were undertaken to determine whether angiotensin II (Ang II) receptors were expressed on these cells. FLTR cell membranes were shown to express high-affinity Ang II receptors having a B-max value of 324 +/- 43 fmol/mg protein and a K-d of 0.36 +/-0.1 nM. In both membranes and intact cells, Ang II, Ang III and the selective AT(1)R antagonist, Losartan, all had a high affinity for the receptor, suggesting that FLTR cells express the AT(1)R subtype. The expression of the hAT(1)R was validated by Northern and Western blot and RT-PCR experiments. In intact FLTR cells, Ang II (100 nM) evoked an increase in intracellular calcium ([Ca2+](i)) and induced hyperplasia. Additionally, our results demonstrated that FLTR cells were readily transfected, and hAT(1)R promoter luciferase constructs exhibited robust promoter activity (i.e. similar to 22-fold increase over pGL3-Basic only). Finally, our results demonstrated that the hAT(1)R gene is differentially regulated in FLTR cells vs. H295-R cells, a human adrenocarcinoma cell line that also abundantly expresses the AT(1)R. Taken together, our results suggest that FLTR cells express functional AT(1)Rs and will provide anexcellent model system in which to investigate hAT(1)R gene regulation. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/01/20 alle ore 18:59:29