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Titolo:
Yeast AMP pathway genes respond to adenine through regulated synthesis of a metabolic intermediate
Autore:
Rebora, K; Desmoucelles, C; Borne, F; Pinson, B; Daignan-Fornier, B;
Indirizzi:
Inst Biochim & Genet Cellulaires, CNRS, UMR 5095, F-33077 Bordeaux, FranceInst Biochim & Genet Cellulaires Bordeaux France F-33077 ordeaux, France
Titolo Testata:
MOLECULAR AND CELLULAR BIOLOGY
fascicolo: 23, volume: 21, anno: 2001,
pagine: 7901 - 7912
SICI:
0270-7306(200112)21:23<7901:YAPGRT>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSCRIPTIONAL ACTIVATORS BAS1; SACCHAROMYCES-CEREVISIAE; ESCHERICHIA-COLI; PHOSPHORIBOSYLPYROPHOSPHATE AMIDOTRANSFERASE; HIS4 TRANSCRIPTION; PROTEIN BAS1P; CLONING; VECTORS; PURINE; REPRESSOR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Daignan-Fornier, B Inst Biochim & Genet Cellulaires, CNRS, UMR 5095, 1 RueCamille St Saens, F-33077 Bordeaux, France Inst Biochim & Genet Cellulaires 1 Rue Camille St Saens Bordeaux France F-33077
Citazione:
K. Rebora et al., "Yeast AMP pathway genes respond to adenine through regulated synthesis of a metabolic intermediate", MOL CELL B, 21(23), 2001, pp. 7901-7912

Abstract

In Saccharomyces cerevisiae, AMP biosynthesis genes (ALE genes) are transcriptionally activated in the absence of extracellular purines by the Bas1p and Bas2p (Pho2p) transcription factors. We now show that expression of theALE genes is low in mutant strains affected in the first seven steps of the pathway, while it is constitutively derepressed in mutant strains affected in later steps. Combined with epistasy studies, these results show that 5'-phosphoribosyl-4-succinocarboxamide-5-aminoimidazole (SAICAR), an intermediate metabolite of the pathway, is needed for optimal activation of the ALE genes. Two-hybrid studies establish that SAICAR is required to promote interaction between Bas1p and Bas2p in vivo, while in vitro experiments suggest that the effect of SAICAR on Bas1p-Bas2p interaction could be indirect. Importantly, feedback inhibition by ATP of Ade4p, catalyzing the first stepof the pathway, appears to regulate SAICAR synthesis in response to adenine availability. Consistently, both ADE4 dominant mutations and overexpression of wild-type ADE4 lead to deregulation of ADE gene expression. We conclude that efficient transcription of yeast AMP biosynthesis genes requires interaction between Bas1p and Bas2p which is promoted in the presence of a metabolic intermediate whose synthesis is controlled by feedback inhibition of Ade4p acting as the purine nucleotide sensor within the cell.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/21 alle ore 01:45:24