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Titolo:
Expression of Leu-19 (CD56, N-CAM) and nitric oxide synthase (NOS) I in denervated and reinnervated human skeletal muscle
Autore:
Gosztonyi, G; Naschold, U; Grozdanovic, Z; Stoltenburg-Didinger, G; Gossrau, R;
Indirizzi:
Free Univ Berlin, Univ Clin Benjamin Franklin, Dept Neuropathol, D-12200 Berlin, Germany Free Univ Berlin Berlin Germany D-12200 opathol, D-12200 Berlin, Germany Free Univ Berlin, Univ Clin Benjamin Franklin, Inst Anat, D-14195 Berlin, Germany Free Univ Berlin Berlin Germany D-14195 st Anat, D-14195 Berlin, Germany
Titolo Testata:
MICROSCOPY RESEARCH AND TECHNIQUE
fascicolo: 3, volume: 55, anno: 2001,
pagine: 187 - 197
SICI:
1059-910X(20011101)55:3<187:EOL(NA>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELL-ADHESION MOLECULE; DUCHENNE MUSCULAR-DYSTROPHY; MONOCLONAL-ANTIBODIES; STRIATED-MUSCLE; NEURAL CELLS; CHICK-EMBRYO; COMPLEX; ANTIGEN; FIBERS; HISTOCHEMISTRY;
Keywords:
muscle pathology; muscle innervation; sarcolemma; dystrophin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Gosztonyi, G Free Univ Berlin, Univ Clin Benjamin Franklin, Dept Neuropathol, Hindenburgdamm 30, D-12200 Berlin, Germany Free Univ Berlin Hindenburgdamm 30 Berlin Germany D-12200 ny
Citazione:
G. Gosztonyi et al., "Expression of Leu-19 (CD56, N-CAM) and nitric oxide synthase (NOS) I in denervated and reinnervated human skeletal muscle", MICROSC RES, 55(3), 2001, pp. 187-197

Abstract

Neural cell adhesion molecule (N-CAM, Leu-19, CD 56) expression appears during muscle fiber regeneration and after denervation. Sarcolemma-associatednitric oxide synthase (NOS) I, however, disappears from denervated myofibers. The dynamics of expression of both proteins were studied in 5 cases of acute/subacute denervation, 28 cases of chronic denervation with and without collateral reinnervation, 5 cases of the intermediate type spinal muscular atrophy (SMA 2), and in 2 normal biopsies. NOS I and its NADPH diaphorase(NADPHd) activity disappeared from the sarcolemma. region shortly after denervation, and before the appearance of denervation. atrophy. N-CAM was found diffusely distributed in the sarcoplasm at the most severe phase of denervation atrophy in the majority of highly atrophic fibers. During reinnervation, NOS I expression remained absent and in part of the cases the target/targetoid phenomenon appeared. In parallel with the increase in volume of the reinnervated muscle fibers, the intensity of N-CAM immunoreactivity decreased progressively. After full restitution of muscle fiber caliber, the target/ targetoid phenomenon and N-CAM immunostaining disappeared completely,and, finally, NOS I reappeared in the sarcolemma region. The sarcolemmal expression of dystrophin and dystrophin-associated proteins was unchanged during denervation. NOS I was completely absent in children with SMA 2, sincethe protein does not appear before 5 years of age in skeletal muscle, while N-CAM was very intensely expressed in the sarcoplasm of highly atrophic denervated muscle fibers. In conclusion, this study suggests that innervation is an important factor for selective gene expression and positioning of NOS I and N-CAM in skeletal muscle and gives practical information for the assessment of the phase and developmental stage of the denervation and reinnervation process. Microsc. Res. Tech. 55:187-197, 2001. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 12:21:36