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Titolo:
Retroviral RNA elements integrate components of post-transcriptional gene expression
Autore:
Boris-Lawrie, K; Roberts, TM; Hull, S;
Indirizzi:
Ohio State Univ, Ctr Retrovirus Res, Columbus, OH 43210 USA Ohio State Univ Columbus OH USA 43210 rovirus Res, Columbus, OH 43210 USA Ohio State Univ, Dept Vet Biosci, Columbus, OH 43210 USA Ohio State Univ Columbus OH USA 43210 Vet Biosci, Columbus, OH 43210 USA Ohio State Univ, Dept Mol Virol Immunol & Med Genet, Columbus, OH 43210 USA Ohio State Univ Columbus OH USA 43210 & Med Genet, Columbus, OH 43210 USA Ohio State Univ, Dept Mol Cellular & Dev Biol, Grad Program, Columbus, OH 43210 USA Ohio State Univ Columbus OH USA 43210 rad Program, Columbus, OH 43210 USA Ohio State Univ, Ctr Comprehens Canc, Columbus, OH 43210 USA Ohio State Univ Columbus OH USA 43210 rehens Canc, Columbus, OH 43210 USA
Titolo Testata:
LIFE SCIENCES
fascicolo: 23, volume: 69, anno: 2001,
pagine: 2697 - 2709
SICI:
0024-3205(20011026)69:23<2697:RREICO>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
IMMUNODEFICIENCY-VIRUS TYPE-1; ROUS-SARCOMA VIRUS; POSTTRANSCRIPTIONAL CONTROL ELEMENT; CONSTITUTIVE TRANSPORT ELEMENT; GAG MESSENGER-RNA; HIV-1 REV PROTEIN; NUCLEAR EXPORT; MUTATIONAL ANALYSIS; DIRECT REPEAT; SECONDARY STRUCTURE;
Keywords:
nuclear export; translation enhancement; spleen necrosis virus RU5; Rev responsive element;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
64
Recensione:
Indirizzi per estratti:
Indirizzo: Boris-Lawrie, K Ohio State Univ, Ctr Retrovirus Res, Columbus, OH 43210 USA Ohio State Univ Columbus OH USA 43210 umbus, OH 43210 USA
Citazione:
K. Boris-Lawrie et al., "Retroviral RNA elements integrate components of post-transcriptional gene expression", LIFE SCI, 69(23), 2001, pp. 2697-2709

Abstract

Retroviruses vary widely in their ability to cause neoplastic transformation or immunodeficiency, and may even lack pathogenicity, but all retroviruses require cytoplasmic expression of intron-containing mRNA. In the cytoplasm, the primary viral transcript has two essential roles as mRNA template for protein synthesis and as genomic RNA for packaging into progeny virions. Cellular proteins are used by the virus to modulate synthesis, processing,and translation of the viral RNA. To subvert the normal RNA processing cascade and achieve nuclear export of intron-containing viral RNA, retroviruses utilize structured RNA elements and viral or cellular protein partners. These nuclear interactions determine the cytoplasmic fate of viral RNAs by facilitating RNA stability, nuclear export, translational efficiency, and even assembly of progeny virions. The HIV Rev responsive element (RRE) and Rev protein have been a informative paradigm for dissection of the process ofeukaryotic RNA nuclear export. Rev is an adapter protein that bridges RRE-containing RNA and the CRM I nuclear export receptor, which delivers intron-containing RNA to a nuclear export pathway typically used for 5s rRNA and protein transport. This review summarizes data indicating that Rev/RRE alsotargets cytoplasmic transcripts to the cytoskeletal polysomes and activates their translational efficiency. The interesting parallel is discussed that genetically simpler retroviruses lack a Rev-like protein and recruit cellular proteins to distinct RNA elements that modulate post-transcriptional gene expression through different export pathways. These pathways include the global mRNA export pathway mediated by Tap, and Tap- and CRM1-independentpathways. The CRM1-independent nuclear export pathway accessed by the spleen necrosis virus post-transcriptional control element is functionally linked to RU5-mediated translational enhancement in the cytoplasm. The simple retroviral post-transcriptional control elements also modulate RNA splicing efficiency, stability, assembly of virions, and subsequent viral egress from the cell. Thus, multiple layers of post-transcriptional control are executed by these retroviral RNA elements, which serve as a compact platform forinteraction with nuclear and possibly cytoplasmic protein partners. Further characterization of the cellular partners and their regulation will be animportant step to full understanding of nuclear-cytoplasmic connections that hardwire post-transcriptional gene expression in eukaryotic cells. (C) 2001 Elsevier Science Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 08:25:54