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Titolo:
Alpha and gamma interferons inhibit herpes simplex virus type 1 infection and spread in epidermal cells after axonal transmission
Autore:
Mikloska, Z; Cunningham, AL;
Indirizzi:
Westmead Hosp, Westmead Millennium Inst, Ctr Virus Res, Westmead, NSW 2145, Australia Westmead Hosp Westmead NSW Australia 2145 , Westmead, NSW 2145, Australia Univ Sydney, Westmead, NSW 2145, Australia Univ Sydney Westmead NSW Australia 2145 ey, Westmead, NSW 2145, Australia
Titolo Testata:
JOURNAL OF VIROLOGY
fascicolo: 23, volume: 75, anno: 2001,
pagine: 11821 - 11826
SICI:
0022-538X(200112)75:23<11821:AAGIIH>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
IFN-GAMMA; LYMPHOCYTE CYTOTOXICITY; ANTEROGRADE TRANSPORT; KERATINOCYTES; LESIONS; IMMUNITY; REPLICATION; EXPRESSION; CLEARANCE; LABIALIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Cunningham, AL Westmead Hosp, Westmead Millennium Inst, Ctr Virus Res, POB214, Westmead,NSW 2145, Australia Westmead Hosp POB 214 Westmead NSW Australia 2145 Australia
Citazione:
Z. Mikloska e A.L. Cunningham, "Alpha and gamma interferons inhibit herpes simplex virus type 1 infection and spread in epidermal cells after axonal transmission", J VIROLOGY, 75(23), 2001, pp. 11821-11826

Abstract

The ability of alpha interferon (IFN-alpha) and IFN-gamma to inhibit transmission of herpes simplex virus type I (HSV-1) from neuronal axon to epidermal cells (ECs), and subsequent spread in these cells was investigated in an in vitro dual-chamber model consisting of human fetal dorsal root ganglia(DRG) innervating autologous skin explants and compared with direct HSV-1 infection of epidermal explants. After axonal transmission from HSV-1-infected DRG neurons, both the number and size of viral cytopathic plaques in ECs was significantly reduced by addition of recombinant IFN-gamma and IFN-alpha to ECs in the outer chamber in a concentration-dependent fashion. Inhibition was maximal when IFNs were added at the same time as the DRG were infected with HSV-1. The mean numbers of plaques were reduced by 52% by IFN-alpha, 36% by IFN-gamma, and by 62% when IFN-alpha and IFN-gamma were combined, and the mean plaque size was reduced by 64, 43, and 72%, respectively. Similar but less-inhibitory effects of both IFNs were observed after direct infection of EC explants, being maximal when IFNs were added simultaneouslyor 6 h before HSV-1 infection. These results show that both IFN-alpha and IFN-gamma can interfere with HSV-1 infection after axonal transmission and subsequent spread of HSV-1 in ECs by a direct antiviral effect. Therefore, both IFN-alpha and -gamma could contribute to the control of HSV-1 spread and shedding in a similar fashion in recurrent herpetic lesions.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 12:13:11