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Titolo:
Control of the differentiation state and function of human epidermal Langerhans cells by cytokines in vitro
Autore:
Prignano, F; Gerlini, G; Fossombroni, V; Pimpinelli, N; Giannotti, B; Nestle, FO; Romagnoli, P;
Indirizzi:
Univ Florence, Dept Anat Histol & Forens Med, Florence, Italy Univ Florence Florence Italy Anat Histol & Forens Med, Florence, Italy Univ Florence, Dept Dermatol, Florence, Italy Univ Florence Florence Italy v Florence, Dept Dermatol, Florence, Italy Careggi Hosp, Tissue Typing Unit, Florence, Italy Careggi Hosp Florence Italy i Hosp, Tissue Typing Unit, Florence, Italy Univ Zurich, Sch Med, Dept Dermatol, Zurich, Switzerland Univ Zurich Zurich Switzerland Med, Dept Dermatol, Zurich, Switzerland
Titolo Testata:
JOURNAL OF THE EUROPEAN ACADEMY OF DERMATOLOGY AND VENEREOLOGY
fascicolo: 5, volume: 15, anno: 2001,
pagine: 433 - 440
SICI:
0926-9959(200109)15:5<433:COTDSA>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
COLONY-STIMULATING FACTOR; NECROSIS-FACTOR-ALPHA; IMMUNOSTIMULATORY DENDRITIC CELLS; ANTIGEN-PRESENTING CELLS; C-KIT LIGAND; T-CELLS; BONE-MARROW; E-CADHERIN; TNF-ALPHA; KERATINOCYTES;
Keywords:
c-kit ligand; granulocyte-macrophage colony-stimulating factor; stem cell factor; tumour necrosis factor-alpha;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Prignano, F Univ Florence, Dept Dermatol Sci, Via Alfani 37, I-50121 Florence, Italy Univ Florence Via Alfani 37 Florence Italy I-50121 nce, Italy
Citazione:
F. Prignano et al., "Control of the differentiation state and function of human epidermal Langerhans cells by cytokines in vitro", J EUR A D V, 15(5), 2001, pp. 433-440

Abstract

Langerhans cells can originate in vitro from immature precursors stimulated with granulocyte macrophage-colony-stimulating factor (GM-CSF), tumour necrosis factor (TNF)-alpha and stem cell factor (SCF). We asked whether these cytokines also control the differentiation state of Langerhans cells within the epidermis and upon leaving this tissue. We harvested sheets of humanepidermis by controlled dispase hydrolysis of keratomes, cultured them in RPMI and 10% fetal calf serum for 48 h and analysed the sheets and the cells migrated spontaneously into the medium, most of which were Langerhans cells containing Birbeck granules. ' By flow cytometry, the intensity of CD1a expression was reduced quite evenly among Langerhans cells migrated from sheets within 48 h. The cells in the sheets underwent loss of dendrites, with a significant reduction in the cell perimeter that was prevented by GM-CSF and TNF-alpha together. Either of these cytokines induced expression of CD18 by cells in the sheets and those in the medium. Moreover, TNF-alpha induced expression of CD54 by cells in the medium, but not by those retained in the sheets, whereas human SCF induced, dose dependently, expression of CD54by cells in the sheets, but not from those in the medium.(2) The proliferation of allogeneic lymphocytes was much higher when stimulating Langerhans cells were harvested from cultures with any cytokine, rather than from cultures without cytokines. We conclude the following: (i) GM-CSF and TNF-alphahelp to maintain full differentiation of Langerhans cells within the epidermis; (ii) cytokine influence on Langerhans cells adhesiveness is in part context dependent; and (iii) pretreatment with cytokines influences positively the number or accessory activity of Langerhans cells on lymphocytes during subsequent mixed leucocyte reaction.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 11:32:44