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Titolo:
MATRIX METALLOPROTEINASE-13 (COLLAGENASE-3) IN HUMAN RHEUMATOID SYNOVIUM
Autore:
LINDY O; KONTTINEN YT; SORSA T; DING YL; SANTAVIRTA S; CEPONIS A; LOPEZOTIN C;
Indirizzi:
UNIV HELSINKI,DEPT MED CHEM,POB 8,SILTAVUORENPENGER 10 A FIN-00014 HELSINKI FINLAND UNIV HELSINKI,CENT HOSP HELSINKI FINLAND INVALID FDN HELSINKI FINLAND UNIV OVIEDO OVIEDO SPAIN
Titolo Testata:
Arthritis and rheumatism
fascicolo: 8, volume: 40, anno: 1997,
pagine: 1391 - 1399
SICI:
0004-3591(1997)40:8<1391:MM(IHR>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
SUBSTRATE-SPECIFICITY; POLYACRYLAMIDE GELS; ISOELECTRIC POINTS; PROTEINS; CELLS; ARTHRITIS; CLONING; CLASSIFICATION; TETRACYCLINES; CRITERIA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
51
Recensione:
Indirizzi per estratti:
Citazione:
O. Lindy et al., "MATRIX METALLOPROTEINASE-13 (COLLAGENASE-3) IN HUMAN RHEUMATOID SYNOVIUM", Arthritis and rheumatism, 40(8), 1997, pp. 1391-1399

Abstract

Objective. To shaw the eventual presence and extent of production of matrix metalloproteinase 13 (MMP-13, or collagenase 3) in rheumatoid synovial tissue samples and extracts, and to assess the inhibition characteristics of recombinant MMP-13, Methods. Immunohistochemical avidin-biotin-peroxidase complex staining/morphometry was used to analyze MMP-13-positive cells in situ, Neutral salt extraction of synovial tissue, electrophoresis of the extract in different buffer systems, and Western blotting were also used, The inhibitory properties of doxcycyline, clodronate, pamidronate, and D-penicillamine for recombinant enzyme were determined with a soluble type II collagen assay, Results. MMP-13was detected in fibroblast- and macrophage-like mononuclear cells in the synovial lining and stroma and in vascular endothelial cells, The overall expression of MMP-13 in these cells in the synovial stroma washigh in rheumatoid arthritis (86 +/- 12%) compared with osteoarthritis (17 +/- 5%) patient samples (P = 0.0027), In a high-pH native electrophoresis gel, inmunoreactivity to anti-MMP-1 and anti-MMP-13 were clearly separated, with anti-MMP-13-immunoreactive material migrating faster than anti-MMP-1-immunoreactive material, Finally, in contrast to MMP-1 and MMP-8, MMP-13 was found to be relatively resistant to the inhibitory effects of doxycycline and clodronate in vitro. Conclusion. Due to its localization in synovial tissue, its substrate profile, increased expression, and relative resistance to known MMP inhibitors, MMP-13 is suggested to play a major role in the pathogenesis of tissue destruction in rheumatoid arthritis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 08:09:00