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Titolo:
The structural dynamics and ligand releasing process after the photodissociation of sperm whale carboxymyoglobin
Autore:
Sakakura, M; Morishima, I; Terazima, M;
Indirizzi:
Kyoto Univ, Grad Sch Sci, Dept Chem, Kyoto 6068502, Japan Kyoto Univ Kyoto Japan 6068502 Sch Sci, Dept Chem, Kyoto 6068502, Japan Kyoto Univ, Grad Sch Engn, Dept Mol Engn, Kyoto 6068501, Japan Kyoto UnivKyoto Japan 6068501 Engn, Dept Mol Engn, Kyoto 6068501, Japan
Titolo Testata:
JOURNAL OF PHYSICAL CHEMISTRY B
fascicolo: 42, volume: 105, anno: 2001,
pagine: 10424 - 10434
SICI:
1520-6106(20011025)105:42<10424:TSDALR>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSIENT GRATING METHOD; SITE-DIRECTED MUTAGENESIS; GLOBAL PROTEIN MOTION; PHOTOACOUSTIC CALORIMETRY; CARBON-MONOXIDE; REACTION VOLUME; GEMINATE RECOMBINATION; PHOTOCHEMICAL-REACTION; ENTHALPY CHANGES; HUMAN MYOGLOBIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
61
Recensione:
Indirizzi per estratti:
Indirizzo: Terazima, M Kyoto Univ, Grad Sch Sci, Dept Chem, Kyoto 6068502, Japan Kyoto Univ Kyoto Japan 6068502 pt Chem, Kyoto 6068502, Japan
Citazione:
M. Sakakura et al., "The structural dynamics and ligand releasing process after the photodissociation of sperm whale carboxymyoglobin", J PHYS CH B, 105(42), 2001, pp. 10424-10434

Abstract

The structural and energy dynamics and molecular diffusion after the photodissociation of sperm whale carboxymyoglobin were investigated by the transient grating (TG) method. The quantitative measurement of the TG signal provides the volume change and released heat within 10 ns (-5 cm(3) mol(-1) and 151 kJ mol(-1), respectively) as well as with a lifetime of 700 ns at 20 degreesC (12 cm(3) mol(-1) and 8 kJ mol(-1)) without any assumption. From the signal intensity with a wide range of grating vector (10(5) to 8 X 10(6)m(-1)), the molecular origin of the 700-ns dynamics is clearly attributed to the ligand escaping process from the protein interior to the solvent. Hence, a four-state model is appropriate to describe the ligand dissociation reaction even at room temperature. Participation of the salt bridge betweenArg45 and the heme 6-propionate side chain as well as the enthalpy change and the structural change of this photodissociation reaction is discussed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/01/20 alle ore 13:29:57