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Titolo:
Thermal lipid order-disorder transitions in mixtures of cationic cholesteryl lipid analogues and dipalmitoyl phosphatidylcholine membranes
Autore:
Ghosh, YK; Indi, SS; Bhattacharya, S;
Indirizzi:
Indian Inst Sci, Dept Organ Chem, Bangalore 560012, Karnataka, India Indian Inst Sci Bangalore Karnataka India 560012 560012, Karnataka, India
Titolo Testata:
JOURNAL OF PHYSICAL CHEMISTRY B
fascicolo: 42, volume: 105, anno: 2001,
pagine: 10257 - 10265
SICI:
1520-6106(20011025)105:42<10257:TLOTIM>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
NUCLEAR-MAGNETIC-RESONANCE; EFFICIENT GENE-TRANSFER; PHASE-EQUILIBRIA; MAMMALIAN-CELLS; AMPHIPHILE; LIPOSOME; RELEASE; DNA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Bhattacharya, S Indian Inst Sci, Dept Organ Chem, Bangalore 560012, Karnataka, India Indian Inst Sci Bangalore Karnataka India 560012 a, India
Citazione:
Y.K. Ghosh et al., "Thermal lipid order-disorder transitions in mixtures of cationic cholesteryl lipid analogues and dipalmitoyl phosphatidylcholine membranes", J PHYS CH B, 105(42), 2001, pp. 10257-10265

Abstract

Two types of cationic cholesteryl amphiphiles, one where the headgroup is attached to the steroid by an ester linkage and the second by an ether linkage, were synthesized. A third type of cholesteryl lipid bearing an oligoethylene glycol segment was also prepared. Each of these synthetic lipids generated vesicle-like aggregates with closed inner aqueous compartments from their aqueous suspensions. We examined their interaction with L-alpha -dipalmitoyl phosphatidylcholine (DPPC) membranes using fluorescence anisotropy,transmission electron microscopy (TENI), and differential scanning calorimetry (DSC). When included in membranes, the synthetic cholesteryl lipids were found to quench the chain motion of the acyl chains of DPPC. This suggests that these cationic cholesteryl derivatives act as filler molecules despite modification at the headgroup level from the molecular structure of natural cholesterol. Careful analyses of DSC and fluorescence anisotropy data suggest that the nature of perturbation induced by each of these cationic cholesterol derivatives is dependent on the details of their molecular structure and provides significant information on the nature of interaction of these derivatives with phospholipid molecules. In general, amphiphiles that support structured water at the interfacial region tend to rigidify the fluid phase more than others. Importantly, these cholesteryl amphiphiles behave less like cholesterol in that their incorporation in DPPC not only abolishes the phase transition but also depresses the phase transition temperature.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/02/20 alle ore 11:36:34