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Titolo:
Subcellular and subsynaptic localization of presynaptic and postsynaptic kainate receptor subunits in the monkey striatum
Autore:
Kieval, JZ; Hubert, GW; Charara, A; Pare, JF; Smith, Y;
Indirizzi:
Emory Univ, Yerkes Reg Primate Res Ctr, Div Neurosci, Atlanta, GA 30322 USA Emory Univ Atlanta GA USA 30322 Ctr, Div Neurosci, Atlanta, GA 30322 USA Emory Univ, Dept Neurol, Atlanta, GA 30322 USA Emory Univ Atlanta GA USA 30322 Univ, Dept Neurol, Atlanta, GA 30322 USA
Titolo Testata:
JOURNAL OF NEUROSCIENCE
fascicolo: 22, volume: 21, anno: 2001,
pagine: 8746 - 8757
SICI:
0270-6474(20011115)21:22<8746:SASLOP>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
METABOTROPIC GLUTAMATE RECEPTORS; SYNAPTIC TRANSMISSION; HUNTINGTONS-DISEASE; SQUIRREL-MONKEY; IMMUNOCYTOCHEMICAL LOCALIZATION; EFFERENT CONNECTIONS; GABAERGIC SYNAPSES; OPIOID RECEPTORS; RAT HIPPOCAMPUS; GLOBUS-PALLIDUS;
Keywords:
Huntington's disease; excitotoxicity; presynaptic receptor; corticostriatal pathway; thalamostriatal pathway; post-embedding immunogold;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
63
Recensione:
Indirizzi per estratti:
Indirizzo: Smith, Y Emory Univ, Yerkes Reg Primate Res Ctr, Div Neurosci, 954 Gatewood Rd NE, Atlanta, GA 30322 USA Emory Univ 954 Gatewood Rd NE Atlanta GA USA30322 , GA 30322 USA
Citazione:
J.Z. Kieval et al., "Subcellular and subsynaptic localization of presynaptic and postsynaptic kainate receptor subunits in the monkey striatum", J NEUROSC, 21(22), 2001, pp. 8746-8757

Abstract

The localization and functions of kainate receptors (KARs) in the CNS are still poorly known. In the striatum, GluR6/7 and KA2 immunoreactivity is expressed presynaptically in a subpopulation of glutamatergic terminals and postsynaptically in dendrites and spines. The goal of this study was to further characterize the subcellular and subsynaptic localization of kainate receptor subunits in the monkey striatum. Immunoperoxidase data reveal that the relative abundance of GluR6/7- and KA2-immunoreactive terminals is homogeneous throughout the striatum irrespective of the differential degree of striatal degeneration in Huntington's disease. Pre-embedding and post-embedding immunogold data indicate that >70% of the presynaptic or postsynaptic GluR6/7 and KA2 labeling is expressed intracellularly. In material stained with the post-embedding immunogold method, approximately one-third of plasmamembrane-bound gold particles labeling in axon terminals and spines is associated with asymmetric synapses, thereby representing synaptic kainate receptor subunits. On the other hand, >60% of the plasmamembrane bound labeling is extrasynaptic. Both GluR6/7 and KA2 labeling in glutamatergic terminals often occurs in clusters of gold particles along the membrane of large vesicular organelles located at various distances from the presynaptic grid. Anterograde labeling from the primary motor cortex or the centromedian thalamic nucleus indicate that both corticostriatal and thalamostriatal terminals express presynaptic GluR6/7 and KA2 immunoreactivity in the postcommissural putamen. In conclusion, these data demonstrate that kainate receptors in the striatum display a pattern of subcellular distribution different fromother ionotropic glutamate receptor subtypes, but consistent with their metabotropic-like functions recently shown in the hippocampus.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 16/07/20 alle ore 19:30:10