Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Kinetic and pharmacological properties of GABA(A) receptors in single thalamic neurons and GABA(A) subunit expression
Autore:
Browne, SH; Kang, J; Akk, G; Chiang, LW; Schulman, H; Huguenard, JR; Prince, DA;
Indirizzi:
Stanford Univ, Dept Neurol & Neurol Sci, Sch Med, Stanford, CA 94305 USA Stanford Univ Stanford CA USA 94305 Sci, Sch Med, Stanford, CA 94305 USA Stanford Univ, Dept Neurobiol, Sch Med, Stanford, CA 94305 USA Stanford Univ Stanford CA USA 94305 biol, Sch Med, Stanford, CA 94305 USA
Titolo Testata:
JOURNAL OF NEUROPHYSIOLOGY
fascicolo: 5, volume: 86, anno: 2001,
pagine: 2312 - 2322
SICI:
0022-3077(200111)86:5<2312:KAPPOG>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
CEREBELLAR GRANULE CELLS; POSTNATAL-DEVELOPMENT; MESSENGER-RNAS; PATCH-CLAMP; RAT-BRAIN; GABA(A)-RECEPTOR SUBTYPES; DESENSITIZATION KINETICS; BENZODIAZEPINE RECEPTORS; CHANNEL PROPERTIES; RETICULAR NEURONS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
52
Recensione:
Indirizzi per estratti:
Indirizzo: Huguenard, JR Stanford Univ, Dept Neurol & Neurol Sci, Sch Med, Stanford, CA 94305 USA Stanford Univ Stanford CA USA 94305 Stanford, CA 94305 USA
Citazione:
S.H. Browne et al., "Kinetic and pharmacological properties of GABA(A) receptors in single thalamic neurons and GABA(A) subunit expression", J NEUROPHYS, 86(5), 2001, pp. 2312-2322

Abstract

Synaptic inhibition in the thalamus plays critical roles in sensory processing and thalamocortical rhythm generation. To determine kinetic, pharmacological, and structural properties of thalamic gamma -aminobutyric acid typeA (GABA(A)) receptors, we used patch-clamp techniques and single-cell reverse transcriptase polymerase chain reaction (RT-PCR) in neurons from two principal rat thalamic nuclei-the reticular nucleus (nRt) and the ventrobasal(VB) complex. Single-channel recordings identified GABA(A) channels with densities threefold higher in VB than nRt neurons, and with mean open time fourfold longer for nRt than VB [14.6 +/-2.5 vs. 3.8 +/-0.7 (SE) ms, respectively]. GABA(A) receptors in nRt and VB cells were pharmacologically distinct. Zn2+ (100 muM) reduced GABA(A) channel activity in VB and nRt by 84 and24%, respectively. Clonazepam (100 nM) increased inhibitory postsynaptic current (IPSC) decay time constants in nRt (from 44.3 to 77.9 ms, P<0.01) but not in VB. Single-cell RT-PCR revealed subunit heterogeneity between nRt and VB cells. VB neurons expressed <alpha>1-alpha3, alpha5, beta1-3, gamma2-3, and delta, while nRt cells expressed alpha3, alpha5, gamma2- 3, and delta. Both cell types expressed more subunits than needed for a single receptor type, suggesting the possibility of GABA(A) receptor heterogeneity within individual thalamic neurons. beta -subunits were not detected in nRt cells, which is consistent with very low levels reported in previous in situ hybridization studies but inconsistent with the expected dependence of functional GABA(A) receptors on beta subunits. Different single-channel open times likely underlie distinct IPSC decay time constants in VB and nRt cells. While we can make no conclusion regarding b subunits, our findings do support a subunits, possibly alpha1 versus alpha3, as structural determinants of channel deactivation kinetics and clonazepam sensitivity. As the gamma2 anddelta subunits previously implicated in Zn2+ sensitivity are both expressed in each cell type, the observed differential Zn2+ actions at VB versus nRt GABA(A) receptors may involve other subunit differences.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 10:02:32