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Titolo:
Robot printing of reverse dot blot arrays for human mutation detection
Autore:
Lappin, S; Cahlik, J; Gold, B;
Indirizzi:
Quest Diagnost, Van Nuys, CA USA Quest Diagnost Van Nuys CA USAQuest Diagnost, Van Nuys, CA USA Beckman Coulter, Fullerton, CA USA Beckman Coulter Fullerton CA USABeckman Coulter, Fullerton, CA USA
Titolo Testata:
JOURNAL OF MOLECULAR DIAGNOSTICS
fascicolo: 4, volume: 3, anno: 2001,
pagine: 178 - 188
SICI:
1525-1578(200111)3:4<178:RPORDB>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE CHAIN-REACTION; BETA-THALASSEMIA MUTATIONS; CYSTIC-FIBROSIS MUTATIONS; DNA; HYBRIDIZATION; GENE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: Gold, B Natl Canc Inst, Lab Genomic Divers, Human Genet Sect, Frederick, MD 21702 USA Natl Canc Inst Frederick MD USA 21702 ct, Frederick, MD 21702 USA
Citazione:
S. Lappin et al., "Robot printing of reverse dot blot arrays for human mutation detection", J MOL DIAGN, 3(4), 2001, pp. 178-188

Abstract

We report on a generally useful, partially automated, human mutation detection method based upon printing moderate density oligonucleotide arrays using a biorobot on activated nylon membranes. The Beckman Biomek 2000 was adapted to this task through fabrication of aluminum membrane filter holders and the development of an addressable Tool Command Language (Tcl) program, which can be invoked through BioScript. During program execution, a robot arm is moved along the x, y, and z axes to expel liquid, without dripping, from disposable barrier pipette tips and then to touch the drops on preactivated membranes. Printed arrays consist of alternating rows of oligonucleotides containing normal and mutant sequences. Hybridization of biotin labeled polymerase chain reaction products derived from human patient genomic DNA samples are visualized using chemiluminescent or chromogenic indicators. This technique allows unequivocal genotyping of 32 mutations at the beta -thalassemia locus (11p15.5) and of 34 mutations and one polymorphism at the cystic fibrosis transconductance membrane regulator locus (7p35).

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/04/20 alle ore 10:47:15