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Titolo:
Comparative detection of measles and rubella IgM and IgG derived from filter paper blood and serum samples
Autore:
Helfand, RF; Keyserling, HL; Williams, I; Murray, A; Mei, J; Moscatiello, C; Icenogle, J; Bellini, WJ;
Indirizzi:
CDC, Resp & Enter Viruses Branch, Ctr Dis Control & Prevent, Atlanta, GA 30333 USA CDC Atlanta GA USA 30333 Ctr Dis Control & Prevent, Atlanta, GA 30333 USA Emory Univ, Sch Med, Dept Pediat, Div Infect Dis Epidemiol & Immunol, Atlanta, GA USA Emory Univ Atlanta GA USA nfect Dis Epidemiol & Immunol, Atlanta, GA USA CDC, Natl Ctr Environm Hlth, Atlanta, GA 30333 USA CDC Atlanta GA USA 30333 C, Natl Ctr Environm Hlth, Atlanta, GA 30333 USA
Titolo Testata:
JOURNAL OF MEDICAL VIROLOGY
fascicolo: 4, volume: 65, anno: 2001,
pagine: 751 - 757
SICI:
0146-6615(200112)65:4<751:CDOMAR>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
IMMUNOGLOBULIN-G; WHOLE-BLOOD; ORAL FLUID; VIRUS; ANTIBODIES; MUMPS; VACCINATION;
Keywords:
EIA; vaccine; vaccination;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: Helfand, RF CDC, Resp & Enter Viruses Branch, Ctr Dis Control & Prevent, 1600 Clifton Rd,Mailstop A-34, Atlanta, GA 30333 USA CDC 1600 Clifton Rd,Mailstop A-34 Atlanta GA USA 30333 333 USA
Citazione:
R.F. Helfand et al., "Comparative detection of measles and rubella IgM and IgG derived from filter paper blood and serum samples", J MED VIROL, 65(4), 2001, pp. 751-757

Abstract

We compared the use of serum and filter paper blood spots as specimen sources for the detection of measles- and rubella-specific IgM and IgG. We collected capillary blood into microtainer tubes and onto filter paper spots from 60 children and 60 healthy adults. The blood was collected from 12-15-month-old children approximately 3 weeks after primary vaccination with measles, mumps, rubella vaccine, and the sample-pairs were tested for measles-specific IgM and IgG antibodies by using a capture antibody EIA and an indirect EIA, respectively. We tested sample-pairs from a subset of participants for rubella-specific IgM and IgG antibodies by using commercially availablecapture IgM (Captia) and indirect IgG (Wampole) assays. The concordance ofresults from serum and filter paper blood spots was high for all assays: 98% for measles IgM, 93% for measles IgG, 94% for rubella IgM, and 93% for rubella IgG, and increased to between 96-100% for all four assays when indeterminate samples were excluded. The correlation coefficients for EIA signals were 0.99 and 0.77 for measles IgM and IgG, respectively, and 0.92 and 0.94 for rubella lgM and IgG, respectively. The cut-off values used for filter paper samples were the same as those used for serum samples for all testsexcept for the rubella IgM assay. The use of filter paper blood spots is apromising future option for the detection of measles- and rubella-specificantibodies. (C) 2001 Wiley-Liss,Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/09/20 alle ore 13:13:56