Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Bacterial lipopolysaccharide induces transforming growth factor beta and hepatocyte growth factor through toll-like receptor 2 in cultured human colon cancer cells
Autore:
Yoshioka, T; Morimoto, Y; Iwagaki, H; Itoh, H; Saito, S; Kobayashi, N; Yagi, T; Tanaka, N;
Indirizzi:
Okayama Univ, Sch Med, Dept Surg 1, Okayama 7008558, Japan Okayama Univ Okayama Japan 7008558 , Dept Surg 1, Okayama 7008558, Japan
Titolo Testata:
JOURNAL OF INTERNATIONAL MEDICAL RESEARCH
fascicolo: 5, volume: 29, anno: 2001,
pagine: 409 - 420
SICI:
0300-0605(200109/10)29:5<409:BLITGF>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTESTINAL EPITHELIAL-CELLS; INNATE IMMUNITY; ENDOTHELIAL-CELLS; GENE-EXPRESSION; BINDING-PROTEIN; C-MET; RAT; INTERLEUKIN-8; LINES; ACTIVATION;
Keywords:
lipopolysaccharide; transforming growth factor; hepatocyte growth factor; toll-like receptor;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Iwagaki, H Okayama Univ, Sch Med, Dept Surg 1, 2-5-1 Shikata Cho, Okayama 7008558, Japan Okayama Univ 2-5-1 Shikata Cho Okayama Japan 7008558 58, Japan
Citazione:
T. Yoshioka et al., "Bacterial lipopolysaccharide induces transforming growth factor beta and hepatocyte growth factor through toll-like receptor 2 in cultured human colon cancer cells", J INT MED R, 29(5), 2001, pp. 409-420

Abstract

This study examined, in human cancer cell lines, the pattern of cytokine production stimulated by lipopolysaccharide (LPS), a major component of the outer surface of Gram-negative bacteria, and characterized the expression pattern of CD14, cell surface LPS receptor antigen, and toll-like receptors (TLRs), which appear to be key regulators of the innate immune response system. Two colon cancer cell lines (DLD and LoVo), a hepatocellular carcinomacell line and a myelomonocytic cell line were incubated with LPS for 0-72 h, and transforming growth factor (TGF) beta1 and beta2, hepatocyte growth factor (HGF) and interleukins 6, 8 and 15 were assayed. The only changes induced by incubation with LPS were significant increases in TGF beta1 production at 12 h, and in HGF production at 72 h, in LPS-stimulated DLD cells, and significant increases in TGF beta2 production after 12 h and in HGF after 72 h in LoVo cells. Using reverse transcriptase-polymerase chain reactionanalysis, expression of CD14 and TLR-2 mRNA was detected in DLD and LoVo cells, and expression of TLR-4 mRNA was detected in PLC/PRF/S and KG-1 cells. These results suggest that LPS induces TGF beta and HGF production mediated by CD14/TLR-2 in cultured human colon cancer cell lines.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 12:40:12