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Titolo:
Antibody fusions with fluorescent proteins: a versatile reagent for profiling protein expression
Autore:
Morino, K; Katsumi, H; Akahori, Y; Iba, Y; Shinohara, M; Ukai, Y; Kohara, Y; Kurosawa, Y;
Indirizzi:
Fujita Hlth Univ, Inst Comprehens Med Sci, Toyoake, Aichi 4701192, Japan Fujita Hlth Univ Toyoake Aichi Japan 4701192 oyoake, Aichi 4701192, Japan Fujita Hlth Univ, Inst Antibody, Toyoake, Aichi 4701192, Japan Fujita HlthUniv Toyoake Aichi Japan 4701192 oyoake, Aichi 4701192, Japan Natl Inst Genet, Mishima, Shizuoka 4118540, Japan Natl Inst Genet MishimaShizuoka Japan 4118540 a, Shizuoka 4118540, Japan
Titolo Testata:
JOURNAL OF IMMUNOLOGICAL METHODS
fascicolo: 1-2, volume: 257, anno: 2001,
pagine: 175 - 184
SICI:
0022-1759(20011101)257:1-2<175:AFWFPA>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
PHAGE DISPLAY TECHNOLOGY; ESCHERICHIA-COLI; FUNCTIONAL EXPRESSION; FV FRAGMENT; CYTOPLASM; INTRABODIES; SYSTEM; INHIBITION; LIBRARIES; STABILITY;
Keywords:
single-chain Fv-CL antibody; green fluorescent protein; red fluorescent proteins; immunostaining; His-tag; POS-1;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Kurosawa, Y Fujita Hlth Univ, Inst Comprehens Med Sci, Toyoake, Aichi 4701192, Japan Fujita Hlth Univ Toyoake Aichi Japan 4701192 i 4701192, Japan
Citazione:
K. Morino et al., "Antibody fusions with fluorescent proteins: a versatile reagent for profiling protein expression", J IMMUNOL M, 257(1-2), 2001, pp. 175-184

Abstract

We developed a system by which antibodies, fused to fluorescent proteins with different wavelengths, can be prepared within a month against various antigens. An antibody library composed of a large number of single-chain Fv-CL fragment was constructed by means of a phage-display system. The constructs were designed to facilitate changing of the protein forms by simple enzyme manipulation. In the present study, we adopted a molecular form of antibody in which a single-chain Fv-CL fragment is fused with a green fluorescent protein (GFP) or red fluorescent protein (RFP). In addition, a His-tag was inserted between CL and GFP (or RFP). We describe the utility of this system using Caenorhabditis elegans embryo as a model. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/07/20 alle ore 22:18:17