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Titolo:
Bcl-2 overexpression decreases BCNU sensitivity of a human glioblastoma line through enhancement of catalase activity
Autore:
Del Bufalo, D; Trisciuoglio, D; Biroccio, A; Marcocci, L; Buglioni, S; Candiloro, A; Scarsella, M; Leonetti, C; Zupi, G;
Indirizzi:
Regina Elena Inst Canc Res, Expt Res Ctr, Expt Chemotherapy Lab, I-00158 Rome, Italy Regina Elena Inst Canc Res Rome Italy I-00158 y Lab, I-00158 Rome, Italy Univ Roma La Sapienza, Biochem Sci Dept A Rossi Fanielli, Rome, Italy UnivRoma La Sapienza Rome Italy Sci Dept A Rossi Fanielli, Rome, Italy Univ Roma La Sapienza, CNR Ctr Mol Biol, Rome, Italy Univ Roma La Sapienza Rome Italy apienza, CNR Ctr Mol Biol, Rome, Italy Regina Elena Inst Canc Res, Dept Pathol, Rome, Italy Regina Elena Inst Canc Res Rome Italy anc Res, Dept Pathol, Rome, Italy
Titolo Testata:
JOURNAL OF CELLULAR BIOCHEMISTRY
fascicolo: 3, volume: 83, anno: 2001,
pagine: 473 - 483
SICI:
0730-2312(2001)83:3<473:BODBSO>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-MALIGNANT GLIOMA; GENE-TRANSFER; O-6-ALKYLGUANINE-DNA ALKYLTRANSFERASE; GLUTATHIONE-PEROXIDASE; SUPEROXIDE DISMUTASES; CELL-DEATH; P53 GENE; EXPRESSION; APOPTOSIS; XENOGRAFTS;
Keywords:
BCNU; glioblastoma; bcl-2; catalase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Del Bufalo, D Regina Elena Inst Canc Res, Expt Res Ctr, Expt Chemotherapy Lab, Via MessidOro 156, I-00158 Rome, Italy Regina Elena Inst Canc Res Via Messi dOro 156 Rome Italy I-00158
Citazione:
D. Del Bufalo et al., "Bcl-2 overexpression decreases BCNU sensitivity of a human glioblastoma line through enhancement of catalase activity", J CELL BIOC, 83(3), 2001, pp. 473-483

Abstract

The aim of this study was to evaluate the role of bcl-2 in 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU) sensitivity of the ADFS human glioblastoma cell line in vitro and in vivo. To this end, the ADFS line expressing a low level of the bcl-2 protein was transfected with a bcl-2 expression vector. Wefound that bcl-2 overexpressing clones were less sensitive to in vitro BCNU treatment than the control clone. Cell cycle analysis demonstrated that while BCNU induced a consistent block in S/G2-M phases of the cell cycle in the control clone, it did not affect the cell cycle phase distribution of the two bcl-2 transfectants. The different sensitivity to BCNU was unrelatedto the ability of bcl-2 to inhibit apoptosis, while bcl-2 appeared to protect bcl-2 transfectants from BCNU toxicity through an increase of catalase activity. The ability of the catalase inhibitor, sodium azide, to increase the BCNU sensitivity of the bcl-2 transfectants to levels of the BCNU-treated control clone substantiated the role of the catalase activity. The effect of bcl-2 in reducing sensitivity to BCNU was also confirmed by in vivo experiments. Xenografts of bcl-2 overexpressing tumors were less sensitive toBCNU treatment than xenografts originating from control cells. J. Cell. Biochem. 83: 473-483, 2001. (C) 2001 Wiley-Liss, Inc.

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Documento generato il 19/09/20 alle ore 06:51:11