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Titolo:
Use of nonionic surfactants for effective supply of phosphatidic acid in serum-free culture of Chinese hamster ovary cells
Autore:
Sakai, K; Hayashi, C; Yamaji, H; Fukuda, H;
Indirizzi:
Kobe Univ, Fac Engn, Dept Sci & Chem Engn, Nada Ku, Kobe, Hyogo 6578501, Japan Kobe Univ Kobe Hyogo Japan 6578501 n, Nada Ku, Kobe, Hyogo 6578501, Japan Kobe Univ, Grad Sch Sci & Technol, Div Mol Sci, Nada Ku, Kobe, Hyogo 6578501, Japan Kobe Univ Kobe Hyogo Japan 6578501 i, Nada Ku, Kobe, Hyogo 6578501, Japan
Titolo Testata:
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
fascicolo: 3, volume: 92, anno: 2001,
pagine: 256 - 261
SICI:
1389-1723(200109)92:3<256:UONSFE>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
LYSOPHOSPHATIDIC ACID; MAMMALIAN-CELLS; GROWTH; PROLIFERATION; FIBROBLASTS;
Keywords:
phosphatidic acid; CHO cells; serum-free culture; nonionic surfactant; Tween 80; Pluronic F-68;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
19
Recensione:
Indirizzi per estratti:
Indirizzo: Yamaji, H Kobe Univ, Fac Engn, Dept Sci & Chem Engn, Nada Ku, 1-1 Rokkodai, Kobe, Hyogo 6578501, Japan Kobe Univ 1-1 Rokkodai Kobe Hyogo Japan 6578501 o 6578501, Japan
Citazione:
K. Sakai et al., "Use of nonionic surfactants for effective supply of phosphatidic acid in serum-free culture of Chinese hamster ovary cells", J BIOSCI BI, 92(3), 2001, pp. 256-261

Abstract

We have previously shown [Sakai et al., J. Biosci. Bioeng., 88, 306-309 (1999)] that exogenously supplied phosphatidic acid (PA) and lysophosphatidicacid (LPA) promoted the growth of Chinese hamster ovary (CHO) cells in serum-free culture. However, the direct addition of high concentrations of these phospholipids alone to the culture medium resulted in the formation of precipitates. We therefore examined the use of two nonionic surfactants, Tween 80 and Pluronic F-68, as a means of supplying PA more effectively to CHOcells in a serum-free culture. A clear dispersion of PA from egg yolk lecithin that could be successfully sterile-filtered was obtained by using Tween 80 or Pluronic F-68. When PA prepared with either of the surfactants was added to serum-free media, precipitation was noticeably reduced. Furthermore, the growth-promoting activity of PA was considerably enhanced by the presence of the surfactants. Since Tween 80 and Pluronic F-68 themselves possessed no growth-stimulating property, it was suggested that the enhanced growth-promoting activity results from the improved availability of PA to the cells. The use of Tween 80 with PA analogues having saturated acyl chains also accelerated cell growth, whereas these PAs showed little growth-promoting activity, due to their poor water-solubility, when added alone.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/04/20 alle ore 11:14:37