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Titolo:
Dendritic cell-tumor coculturing vaccine can induce antitumor immunity through both NK and CTL interaction
Autore:
Kim, KD; Choi, SC; Kim, A; Choe, YK; Choe, IS; Lim, JS;
Indirizzi:
Korea Res Inst Biosci & Biotechnol, Cell Biol Lab, Taejon 305600, South Korea Korea Res Inst Biosci & Biotechnol Taejon South Korea 305600 South Korea
Titolo Testata:
INTERNATIONAL IMMUNOPHARMACOLOGY
fascicolo: 12, volume: 1, anno: 2001,
pagine: 2117 - 2129
SICI:
1567-5769(200111)1:12<2117:DCCVCI>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
NATURAL-KILLER-CELLS; CYTOTOXIC T-LYMPHOCYTES; IN-VITRO; MURINE TUMORS; ANTIGEN; PEPTIDE; INNATE; VIVO; RNA; IMMUNIZATION;
Keywords:
dendritic cells (DC); natural killer (NK) cells; CTL; antitumor activity; IFN-gamma;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Lim, JS Korea Res Inst Biosci & Biotechnol, Cell Biol Lab, Yusung POB 115,Taejon 305600, South Korea Korea Res Inst Biosci & Biotechnol Yusung POB 115 Taejon South Korea 305600
Citazione:
K.D. Kim et al., "Dendritic cell-tumor coculturing vaccine can induce antitumor immunity through both NK and CTL interaction", INT IMMUNO, 1(12), 2001, pp. 2117-2129

Abstract

Immunization of dendritic cells (DC) pulsed with tumor antigen can activate tumor-specific cytotoxic T lymphocytes (CTL) that are responsible for protection and regression. We show here that immunization with bone marrow-derived DC cocultured with tumor cells can induce a protective immunity against challenges to viable tumor cells. In this study, we further investigated the mechanism by which the antitumor activity was induced. Immunization of mice with DC cocultured with murine colon carcinoma, CT-26 cells, augmentedCTL activity against the tumor cells. Concomitantly, an increase in natural killer (NK) cell activity was also detected in the same mice. When DC were fixed with paraformaldehyde prior to coculturing with tumor cells, most of the CTL and NK cell activity diminished, indicating that DC are involved in the process of presenting the tumor antigen(s) to CTL. NK cell depletionin vivo produced markedly low tumor-specific CTL activity responsible for tumor prevention. In addition, RT-PCR analysis confirmed the high expression of INF-gamma mRNA in splenocytes after vaccination with DC cocultured with tumors, but low expression in splenocytes from NK-depleted mice. Most importantly, the tumor protective effect rendered to DC by the coculturing with CT-26 cells was not observed in NK-depleted mice, which suggests that DC can induce an antitumor immune response by enhancing NK cell-dependent CTL activation. Collectively, our results indicate that NK cells are required during the priming of cytotoxic T-cell response by DC-based tumor vaccine and seem to delineate a mechanism by which DC vaccine can provide the desiredimmunity, (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 17:12:08