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Titolo:
Cortisone counteracts apoptosis-inducing effect of cortisol in human peripheral-blood mononuclear cells
Autore:
Hirano, T; Horigome, A; Takatani, M; Oka, K;
Indirizzi:
Tokyo Univ Pharm & Life Sci, Sch Pharm, Dept Clin Pharmacol, Hachioji, Tokyo 1920392, Japan Tokyo Univ Pharm & Life Sci Hachioji Tokyo Japan 1920392o 1920392, Japan
Titolo Testata:
INTERNATIONAL IMMUNOPHARMACOLOGY
fascicolo: 12, volume: 1, anno: 2001,
pagine: 2109 - 2115
SICI:
1567-5769(200111)1:12<2109:CCAEOC>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHRONIC-RENAL-FAILURE; 11-BETA-HYDROXYSTEROID DEHYDROGENASE; T-LYMPHOCYTES; GLUCOCORTICOID-RECEPTORS; IN-VITRO; PHARMACODYNAMICS; PSORIASIS; EFFICACY; TISSUES; ENZYME;
Keywords:
glucocorticoid; oxidized glucocorticoid; attenuation of apoptosis; mitogen-activated lymphocytes; human peripheral-blood mononuclear cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Hirano, T Tokyo Univ Pharm & Life Sci, Sch Pharm, Dept Clin Pharmacol, 1432-1 Horinouchi, Hachioji, Tokyo 1920392, Japan Tokyo Univ Pharm & Life Sci 1432-1 Horinouchi Hachioji Tokyo Japan 1920392
Citazione:
T. Hirano et al., "Cortisone counteracts apoptosis-inducing effect of cortisol in human peripheral-blood mononuclear cells", INT IMMUNO, 1(12), 2001, pp. 2109-2115

Abstract

Glucocorticoids (GCs) have been considered to regulate immune cell systemsthrough induction of apoptosis in thymocytes and mature peripheral-blood lymphocytes. Here we report that apoptosis induced by cortisol in mitogen-activated peripheral-blood mononuclear cells (PBMC) is suppressed by cortisone, an oxidized metabolite of cortisol. Apoptosis in PBMCs is quantified by a cell death ELISA procedure, which can specifically detect fragmented DNA. Cortisol induced PBMC-apoptosis at concentrations more than 10 ng/ml (28 nM) in concanavalin A-stimulated PBMCs and cortisone suppressed this apoptosis at a concentration range of 1-10,000 ng/ml (2.8-28,000 nM) dose-dependently. Prednisone, a synthetic oxidized-GC, also suppressed the apoptosis-inducing effect of cortisol in a dose-dependent manner. Suppression of cortisol-induced apoptosis by cortisone was consistently observed in PBMCs derivedfrom 16 healthy subjects. Examination for inhibitory activities of the steroids against [H-3]dexamethasone binding to PBMCs suggested that cortisone can bind cellular GC-receptors (GC-Rs), but the affinity of cortisone to GCRs is 1/30 or less than that of cortisol. The results raised a possible role of cortisone in cortisol-mediated regulation of apoptosis in activated human PBMCs. The counteracting action of cortisone against cortisol-induced apoptosis may take place partially through intervention of GC-receptors (GC-Rs), but may also be due to unknown pathway(s) different from those mediated by cellular GC-Rs. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/01/20 alle ore 03:19:08