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Titolo:
Ecdysone-inducible foreign gene expression in stably-transformed lepidopteran insect cells
Autore:
Tomita, S; Kawai, Y; Woo, SD; Kamimura, M; Iwabuchi, K; Imanishi, S;
Indirizzi:
Natl Inst Sericultural & Entomol Sci, Dept Insect Genet & Breeding, Tsukuba, Ibaraki 3058634, Japan Natl Inst Sericultural & Entomol Sci Tsukuba Ibaraki Japan 3058634 Japan Natl Inst Sericultural & Entomol Sci, Dept Sericulture, Tsukuba, Ibaraki 3058634, Japan Natl Inst Sericultural & Entomol Sci Tsukuba Ibaraki Japan 3058634 Japan Tokyo Univ Agr & Technol, Fac Agr, Fuchu, Tokyo 1830054, Japan Tokyo Univ Agr & Technol Fuchu Tokyo Japan 1830054 , Tokyo 1830054, Japan
Titolo Testata:
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
fascicolo: 9, volume: 37, anno: 2001,
pagine: 564 - 571
SICI:
1071-2690(200110)37:9<564:EFGEIS>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
RECEPTOR B1 ISOFORM; BOMBYX-MORI; TOBACCO HORNWORM; MESSENGER-RNAS; MANDUCA-SEXTA; CLONING; SILKWORM; ELEMENT; 20-HYDROXYECDYSONE; TRANSCRIPTION;
Keywords:
reporter assay; ecdysone-response element; ecdysone agonist; ecdysone receptor;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Tomita, S Natl Inst Sericultural & Entomol Sci, Dept Insect Genet & Breeding, 1-2 Ohwashi, Tsukuba, Ibaraki 3058634, Japan Natl Inst Sericultural & Entomol Sci 1-2 Ohwashi Tsukuba Ibaraki Japan 3058634
Citazione:
S. Tomita et al., "Ecdysone-inducible foreign gene expression in stably-transformed lepidopteran insect cells", IN VITRO-AN, 37(9), 2001, pp. 564-571

Abstract

Cultured cell lines that can be stably transformed with inducible gene constructs could prove extremely valuable for the continuous and economical production of recombinant proteins. Toward this goal, we have established 11 clones (designated NISES-BoMo-DK1 to 11) from a previously reported silkworm cell line, NISES-BoMo-DZ. Nine of these clonal lines showed a distinct morphological change, i.e., cell aggregation, in response to treatment with ImuM 20-hydroxyecdysone (20E). DK10 cells transfected with various reporterassay plasmids under optimal conditions (i.e., 20-30% transfection efficiency) showed inducibility of gene expression by 20E. The 20E treatment of the prototypical DK10 cells resulted in a simultaneous, transient increase ofthe nuclear ecdysone (E) receptor levels. Further. this inducibility was also observed in a DK10 cell line stably transformed with the reporter plasmid that carries the hygromycin-resistance gene. This offers an opportunity to achieve efficient, continuous production of recombinant proteins. It could also allow high throughput screening for potential E agonists.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 08:57:21