Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
hGFAP-cre transgenic mice for manipulation of glial and neuronal function in vivo
Autore:
Zhuo, L; Theis, M; Alvarez-Maya, I; Brenner, M; Willecke, K; Messing, A;
Indirizzi:
Univ Wisconsin, Waisman Ctr, Dept Pathobiol Sci, Madison, WI 53705 USA Univ Wisconsin Madison WI USA 53705 Pathobiol Sci, Madison, WI 53705 USA Univ Wisconsin, Sch Vet Med, Madison, WI 53706 USA Univ Wisconsin MadisonWI USA 53706 n, Sch Vet Med, Madison, WI 53706 USA Univ Bonn, Abt Mol Genet, Genet Inst, D-5300 Bonn, Germany Univ Bonn Bonn Germany D-5300 ol Genet, Genet Inst, D-5300 Bonn, Germany Univ Alabama, Dept Neurobiol, Birmingham, AL 35294 USA Univ Alabama Birmingham AL USA 35294 Neurobiol, Birmingham, AL 35294 USA Univ Alabama, Dept Phys Med & Rehabil, Birmingham, AL 35294 USA Univ Alabama Birmingham AL USA 35294 & Rehabil, Birmingham, AL 35294 USA
Titolo Testata:
GENESIS
fascicolo: 2, volume: 31, anno: 2001,
pagine: 85 - 94
SICI:
1526-954X(200110)31:2<85:HTMFMO>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
FIBRILLARY ACIDIC PROTEIN; PERISINUSOIDAL STELLATE CELLS; SITE-SPECIFIC RECOMBINATION; CENTRAL-NERVOUS-SYSTEM; NEURAL STEM-CELLS; MUTANT MICE; CHOROID-PLEXUS; SCHWANN-CELLS; GENE; GFAP;
Keywords:
astrocyte; development; central nervous system; gene targeting;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
55
Recensione:
Indirizzi per estratti:
Indirizzo: Messing, A Univ Wisconsin, Waisman Ctr, Dept Pathobiol Sci, Room 713,1500 Highland Ave, Madison, WI 53705 USA Univ Wisconsin Room 713,1500 Highland Ave Madison WI USA 53705
Citazione:
L. Zhuo et al., "hGFAP-cre transgenic mice for manipulation of glial and neuronal function in vivo", GENESIS, 31(2), 2001, pp. 85-94

Abstract

With the goal of performing astrocyte-specific modification of genes in the mouse, we have generated a transgenic line expressing Cre recombinase under the control of the human glial fibrillary acidic protein (hGFAP) promoter. Activity was monitored by crossing the hGFAP-cre transgenics with eitherof two reporter lines carrying a lacZ gene whose expression requires excision of l flanked stop sequences. We found that lacZ expression was primarily limited to the central nervous system, but therein was widespread in neurons and ependyma. Cell types within the brain that notably failed to activate lacZ expression included Purkinje neurons of the cerebellum and choroid plexus epithelium. Onset of Cre expression began in the forebrain by e13.5,suggesting that the hGFAP promoter is active in a multi-potential neural stem cell. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/20 alle ore 22:08:24