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Titolo:
SNRPN methylation patterns in germ cell tumors as a reflection of primordial germ cell development
Autore:
Bussey, KJ; Lawce, HJ; Himoe, E; Shu, XO; Heerema, NA; Perlman, EJ; Olson, SB; Magenis, RE;
Indirizzi:
Oregon Hlth Sci Univ, Cytogenet Res Lab, Dept Mol & Med Genet, Portland, OR 97201 USA Oregon Hlth Sci Univ Portland OR USA 97201 Genet, Portland, OR 97201 USA Vanderbilt Univ, Dept Med, Nashville, TN USA Vanderbilt Univ Nashville TNUSA rbilt Univ, Dept Med, Nashville, TN USA Parker Hughes Inst, Dept Genet, St Paul, MN USA Parker Hughes Inst St Paul MN USA ghes Inst, Dept Genet, St Paul, MN USA Johns Hopkins Univ, Dept Pathol, Baltimore, MD 21205 USA Johns Hopkins Univ Baltimore MD USA 21205 Pathol, Baltimore, MD 21205 USA Childrens Canc Grp, Arcadia, CA USA Childrens Canc Grp Arcadia CA USAChildrens Canc Grp, Arcadia, CA USA Oregon Hlth Sci Univ, Child Dev Rehabil Ctr, Portland, OR 97201 USA OregonHlth Sci Univ Portland OR USA 97201 il Ctr, Portland, OR 97201 USA
Titolo Testata:
GENES CHROMOSOMES & CANCER
fascicolo: 4, volume: 32, anno: 2001,
pagine: 342 - 352
SICI:
1045-2257(200112)32:4<342:SMPIGC>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
PRADER-WILLI-SYNDROME; ACUTE LYMPHOBLASTIC-LEUKEMIA; BIALLELIC EXPRESSION; IN-UTERO; IMPRINTED EXPRESSION; GENE REARRANGEMENTS; ANGELMAN SYNDROMES; IDENTICAL-TWINS; DNA METHYLATION; PARENTAL ORIGIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Magenis, RE Oregon Hlth Sci Univ, Cytogenet Res Lab, Dept Mol & Med Genet,3181 SW SamJackson Pk Rd,MP350, Portland, OR 97201 USA Oregon Hlth Sci Univ 3181 SW Sam Jackson Pk Rd,MP350 Portland OR USA 97201
Citazione:
K.J. Bussey et al., "SNRPN methylation patterns in germ cell tumors as a reflection of primordial germ cell development", GENE CHROM, 32(4), 2001, pp. 342-352

Abstract

Studies examining altered imprinted gene expression in cancer compare the observed expression pattern to the normal expression pattern for a given tissue of origin, usually the somatic expression pattern for the imprinted gene. Germ cell tumors (GCTs), however, require a developmental stage-dependent comparison. To explore using methylation as an indicator of germ cell development, we determined the pattern of methylation at the 5' untranslated region of SNRPN in 89 GCTs from both children and adults. Fifty-one of 84 tumors (60.7%) (12/30 (40%) of cultured pediatric GCTs, 23/36 (63.9%) of frozen adult GCTs, and 16/23 (69.5%) of frozen pediatric GCTs, with five samples having results from both cultured and uncultured material) demonstrated a nonsomatic methylation pattern after dual digestion with Xbal, Notl, and Southern blot analysis. In contrast, only 2 of 18 (11%) control samples (16non-GCTs and 2 normal ovaries) exhibited a nonsomatic pattern. In both cases, the result was shown to be due to copy number differences between maternal and paternal homologs, unlike the GCTs in which there was no evidence of an uneven homolog number. A comparison of the data for only the gonadal GCTs and the control data showed a highly significant difference in the proportion of tumors with methylation alterations at this locus (P=0.0000539). Since there is no published evidence of the involvement of SNRPN methylation changes in the development of malignancy, the data suggest that the methylation pattern of SNRPN in GCTs reflects that of the primordial germ cell giving rise to the tumor. (C) 2001 Wiley-Liss, Inc.

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Documento generato il 01/06/20 alle ore 01:36:27