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Titolo:
Flanking regulatory sequences of the locus encoding the murine GDNF receptor, c-ret, directs Lac Z (beta-galactosidase) expression in developing somatosensory system
Autore:
Sukumaran, M; Waxman, SG; Wood, JN; Pachnis, V;
Indirizzi:
Univ Coll London, Dept Biol, Mol Nocicept Lab, London WC1E 6BT, England Univ Coll London London England WC1E 6BT t Lab, London WC1E 6BT, England Natl Inst Med Res, Div Dev Neurobiol, London NW7 1AA, England Natl Inst Med Res London England NW7 1AA robiol, London NW7 1AA, England Yale Univ, Sch Med, Dept Neurol, New Haven, CT 06510 USA Yale Univ New Haven CT USA 06510 ed, Dept Neurol, New Haven, CT 06510 USA
Titolo Testata:
DEVELOPMENTAL DYNAMICS
fascicolo: 3, volume: 222, anno: 2001,
pagine: 389 - 402
SICI:
1058-8388(200111)222:3<389:FRSOTL>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENTERIC NERVOUS-SYSTEM; DORSAL ROOT-GANGLIA; MICE LACKING GDNF; NEUROBLASTOMA CELL-LINES; NEOPLASIA TYPE 2B; NEUROTROPHIC FACTOR; TYROSINE KINASE; SENSORY NEURONS; TRANSGENIC MICE; DNA METHYLATION;
Keywords:
c-ret; GDNF receptor; nociceptors; neurotrophic factors; promoter analysis; transgenesis; sensory neurone development;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
85
Recensione:
Indirizzi per estratti:
Indirizzo: Sukumaran, M Univ Coll London, Dept Anat & Dev Biol, Medawar Bldg,Gower St, London WC1E6BT, England Univ Coll London Medawar Bldg,Gower St London England WC1E 6BT
Citazione:
M. Sukumaran et al., "Flanking regulatory sequences of the locus encoding the murine GDNF receptor, c-ret, directs Lac Z (beta-galactosidase) expression in developing somatosensory system", DEV DYNAM, 222(3), 2001, pp. 389-402

Abstract

RET forms the catalytic component within the receptor complex that transmits signals from the GDNF family of neurotrophic factors. To study the mechanisms regulating the cell-type specific expression of this gene, we have cloned and characterised the murine c-ret locus. A cosmid contig comprising approximately 60 kb of the mouse genome encompassing the entire structural gene and flanking sequences have been isolated and the transcription initiation site identified and promoter characterised. The murine c-ret promoter lacks a TATA initiation motif and has GC enriched DNA sequences reminiscent of CpG islands. Analysis of transgenic mice lines bearing the Lac Z (beta -galactosidase) reporter gene under the control of 5' flanking sequences show modularity in the organisation of cis-regulatory domains within the locus. Cloned 5' flanking sequences comprise a distal regulatory domain directing Lac Z expression at the primitive streak, lateral mesoderm and facial ganglia and a proximal sensory neurones specific regulatory domain inducing Lac Z expression primarily within the developing somatosensory system. The spatial and temporal progression of transgene expression precisely recapitulates endogenous gene expression in developing sensory ganglia including its induction in postnatal Isolectin B4 binding nociceptive neurones. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 04:39:01