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Titolo:
Vanadate disrupts mammary gland development in whole organ culture
Autore:
Gallo-Hendrikx, E; Murray, SA; Vonderhaar, BK; Xiao, ZXJ;
Indirizzi:
Boston Univ, Sch Med, Dept Med, Boston, MA 02118 USA Boston Univ Boston MA USA 02118 , Sch Med, Dept Med, Boston, MA 02118 USA Boston Univ, Sch Med, Dept Biochem, Boston, MA 02118 USA Boston Univ Boston MA USA 02118 h Med, Dept Biochem, Boston, MA 02118 USA NCI, Tumor Immunol & Biol Lab, Mol & Cellular Endocrinol Sect, Bethesda, MD 20892 USA NCI Bethesda MD USA 20892 ellular Endocrinol Sect, Bethesda, MD 20892 USA
Titolo Testata:
DEVELOPMENTAL DYNAMICS
fascicolo: 3, volume: 222, anno: 2001,
pagine: 354 - 367
SICI:
1058-8388(200111)222:3<354:VDMGDI>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-TYROSINE PHOSPHATASES; PROGRAMMED CELL-DEATH; HUMAN-BREAST-CANCER; EPITHELIAL-CELLS; EXTRACELLULAR-MATRIX; VANADIUM COMPOUNDS; GROWTH-HORMONE; DNA-SYNTHESIS; NEU ONCOGENE; EXPRESSION;
Keywords:
vanadate; protein tyrosine phosphatases; development; mammary gland; whole organ culture;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
52
Recensione:
Indirizzi per estratti:
Indirizzo: Xiao, ZXJ Boston Univ, Sch Med, Dept Med, 88 E Newton St,E605, Boston, MA 02118 USA Boston Univ 88 E Newton St,E605 Boston MA USA 02118 MA 02118 USA
Citazione:
E. Gallo-Hendrikx et al., "Vanadate disrupts mammary gland development in whole organ culture", DEV DYNAM, 222(3), 2001, pp. 354-367

Abstract

Protein tyrosine kinases and phosphatases are signaling molecules involvedin all aspects of development, including proliferation, differentiation, and apoptosis. How disruption of protein tyrosine phosphatase affects mammary gland development is not entirely clear. We examined the effects of sodium vanadate, which is known to primarily inhibit tyrosine phosphatases, in mouse mammary gland development in whole organ culture. Mammary epithelial differentiation was effectively inhibited by vanadate in a dose-dependent manner as indicated by lack of epithelial alveoli compared to the contralateral nontreated gland controls. Mammary glands in the differentiation medium after four days in the presence of vanadate did not differentiate into alveoli. Instead, they exhibited prominent terminal end buds and lost the distinctive epithelial structures. The inhibitory effect of vanadate on mammary epithelial cell differentiation was irreversible after one day of treatment. Immunohistochemical staining for PCNA (Proliferating Cell Nuclear Antigen) showed that vanadate-treated glands exhibited elevated proliferation signals in the differentiation medium. Expression of beta -casein protein in the vanadate-treated glands decreased dramatically and progressively. Short-term exposure (up to 72 hours) of mammary glands to vanadate resulted in an increase in mammary epithelial cell density and loss of organization of themammary structures. TUNEL assay of mammary glands with prolonged exposure to vanadate revealed widespread apoptosis. Furthermore, some cells were still proliferating or expressing beta -casein after prolonged exposure to vanadate. Taken together, these data indicate that vanadate treatment blocks mammary epithelial cell differentiation and promotes abnormal proliferation and apoptosis, likely through the inhibition of protein tyrosine phosphatase-mediated signaling. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 12:29:28