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Titolo:
Proinflammatory cytokines regulate the gene expression of hyaluronic acid synthetase in cultured rabbit synovial membrane cells
Autore:
Tanimoto, K; Ohno, S; Fujimoto, K; Honda, K; Ijuin, C; Tanaka, N; Doi, T; Nakahara, M; Tanne, K;
Indirizzi:
Hiroshima Univ, Fac Dent, Dept Orthodont, Minami Ku, Hiroshima 7348553, Japan Hiroshima Univ Hiroshima Japan 7348553 nami Ku, Hiroshima 7348553, Japan Hiroshima Univ, Fac Dent, Dept Biochem, Minami Ku, Hiroshima 7348553, Japan Hiroshima Univ Hiroshima Japan 7348553 nami Ku, Hiroshima 7348553, Japan
Titolo Testata:
CONNECTIVE TISSUE RESEARCH
fascicolo: 3, volume: 42, anno: 2001,
pagine: 187 - 195
SICI:
0300-8207(2001)42:3<187:PCRTGE>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR-NECROSIS-FACTOR; FACTOR-KAPPA-B; MOLECULAR-CLONING; RHEUMATOID-ARTHRITIS; FACTOR-ALPHA; SYNTHASE; FIBROBLASTS; INTERLEUKIN-1-BETA; MACROPHAGES; FLUID;
Keywords:
quantitative real-time PCR; HAS; IL-1 beta; TNF-alpha; IFN-gamma;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Tanimoto, K Hiroshima Univ, Fac Dent, Dept Orthodont, Minami Ku, 1-2-3 Kasumi, Hiroshima 7348553, Japan Hiroshima Univ 1-2-3 Kasumi Hiroshima Japan 7348553 53, Japan
Citazione:
K. Tanimoto et al., "Proinflammatory cytokines regulate the gene expression of hyaluronic acid synthetase in cultured rabbit synovial membrane cells", CONNECT TIS, 42(3), 2001, pp. 187-195

Abstract

To elucidate the mechanism of accumulation and fragmentation of hyaluronicacid (HA) under inflammatory conditions, we investigated the effect of proinflammatory cytokines on hyaluronic acid synthetase (HAS) mRNA expression using cultured rabbit synovial membrane cells. HASs mRNA levels were determined by real-time PCR. HAS2 mRNA expression was maximally enhanced 3.3- and2.8-fold after 3-hour stimulation with IL-1 beta (1 ng/ml) and after 1-hour stimulation with TNF-alpha (10 ng/ml). HAS3 mRNA expression was increasedby a maximum of 4.3 times after 3-hour stimulation with IL-1 beta (10 ng/ml), whereas 1-hour stimulation with TNF-alpha (10 ng/ml) and IFN-gamma (10 ng/ml) induced around a 2.5-fold increase in HAS3 mRNA. Although IFN-gamma (1+100 ng/ml) alone showed little effect on HAS2 mRNA expression, the effect was synergized by combined with both IL-1 beta and TNF-alpha, substantially increasing HAS2 mRNA expression. These results suggest that proinflammatory cytokines regulate the HAS expression. and consequently may contribute to the accumulation and fragmentation of HA.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 00:31:43