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Titolo:
Tuberculin skin testing compared with T-cell responses to Mycobacterium tuberculosis-specific and nonspecific antigens for detection of latent infection in persons with recent tuberculosis contact
Autore:
Arend, SM; Engelhard, ACF; Groot, G; de Boer, K; Andersen, P; Ottenhoff, THW; van Dissel, JT;
Indirizzi:
Leiden Univ, Med Ctr, Dept Infect Dis, NL-2300 RC Leiden, Netherlands Leiden Univ Leiden Netherlands NL-2300 RC NL-2300 RC Leiden, Netherlands Leiden Univ, Med Ctr, Dept Immunohematol & Blood Transfus, NL-2300 RC Leiden, Netherlands Leiden Univ Leiden Netherlands NL-2300 RC NL-2300 RC Leiden, Netherlands Municipal Hlth Dept Zaanstreek & Waterland, Dept TB Control, Zaandam, Netherlands Municipal Hlth Dept Zaanstreek & Waterland Zaandam Netherlands herlands Football Club Volendam, Volendam, Netherlands Football Club Volendam Volendam Netherlands ndam, Volendam, Netherlands Statens Serum Inst, Dept TB Immunol, DK-2300 Copenhagen, Denmark Statens Serum Inst Copenhagen Denmark DK-2300 K-2300 Copenhagen, Denmark
Titolo Testata:
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY
fascicolo: 6, volume: 8, anno: 2001,
pagine: 1089 - 1096
SICI:
1071-412X(200111)8:6<1089:TSTCWT>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
DELAYED-TYPE HYPERSENSITIVITY; CULTURE FILTRATE PROTEIN-10; PROTECTIVE IMMUNITY; BOVIS BCG; ESAT-6 ANTIGEN; DIAGNOSIS; DISEASE; IDENTIFICATION; PEPTIDES; VACCINES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Arend, SM Leiden Univ, Med Ctr, Dept Infect Dis, C5P,POB 9600, NL-2300 RC Leiden, Netherlands Leiden Univ C5P,POB 9600 Leiden Netherlands NL-2300 RCherlands
Citazione:
S.M. Arend et al., "Tuberculin skin testing compared with T-cell responses to Mycobacterium tuberculosis-specific and nonspecific antigens for detection of latent infection in persons with recent tuberculosis contact", CL DIAG LAB, 8(6), 2001, pp. 1089-1096

Abstract

The tuberculin skin test (TST) is used for the identification of latent tuberculosis (TB) infection (LTBI) but lacks specificity in Mycobacterium bovis BCG-vaccinated individuals, who constitute an increasing proportion of TB patients and their contacts from regions where TB is endemic. In previousstudies, T-cell responses to ESAT-6 and CFP-10, M. tuberculosis-specific antigens that are absent from BCG, were sensitive and specific for detectionof active TB. We studied 44 close contacts of ia patient with smear-positive pulmonary TB and compared the standard screening procedure for LTBI by TST or chest radiographs with T-cell responses to M. tuberculosis-specific and nonspecific antigens. Peripheral blood mononuclear cells were coculturedwith ESAT-6, CFP-10, TB10.4 (each as recombinant antigen and as a mixture of overlapping synthetic peptides), M. tuberculosis sonicate, purified protein derivative (PPD), and short-term culture filtrate, using gamma interferon production as the response measure. LTBI screening was by TST in 36 participants and by chest radiographs in 8 persons. Nineteen contacts were categorized as TST negative, 12 were categorized as TST positive, and 5 had indeterminate TST results. Recombinant antigens and peptide mixtures gave similar results. Responses to TB10.4 were neither sensitive nor specific for LTBI. T-cell responses to ESAT-6 and CFP-10 were less sensitive for detectionof LTBI than those to PPD (67 versus 100%) but considerably more specific (100 versus 72%). The specificity of the TST or in vitro responses to PPD will be even less when the proportion of BCG-vaccinated persons among TB contacts evaluated for LTBI increases.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 06:40:50