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Titolo:
SGK and 14-3-3 protein are involved in the serine/threonine phosphorylation mechanism for TPO/MPL signal transduction
Autore:
Feng, LB; Yang, LM; Zhou, WG; Huang, L; Wan, M; Zhao, SY; Li, CB;
Indirizzi:
Fudan Univ, Inst Genet, State Key Lab Genet Engn, Shanghai 200433, PeoplesR China Fudan Univ Shanghai Peoples R China 200433 anghai 200433, PeoplesR China
Titolo Testata:
CHINESE SCIENCE BULLETIN
fascicolo: 22, volume: 46, anno: 2001,
pagine: 1880 - 1885
SICI:
1001-6538(200111)46:22<1880:SA1PAI>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN BLOOD-PLATELETS; THROMBOPOIETIN; KINASE; PATHWAY; BINDING; SERUM;
Keywords:
thrombopioetin receptor c-Mpl; yeast two-hybrid system; SGK; 14-3-3 protein; interaction;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
14
Recensione:
Indirizzi per estratti:
Indirizzo: Li, CB Fudan Univ, Inst Genet, State Key Lab Genet Engn, Shanghai 200433, PeoplesR China Fudan Univ Shanghai Peoples R China 200433 200433, PeoplesR China
Citazione:
L.B. Feng et al., "SGK and 14-3-3 protein are involved in the serine/threonine phosphorylation mechanism for TPO/MPL signal transduction", CHIN SCI B, 46(22), 2001, pp. 1880-1885

Abstract

Thrombopioetin (TPO), the critical regulator of platelet production, acts by binding to its cell surface receptor, c-Mpl. Yeast two-hybrid screening was performed to isolate the proteins interacting with the cytoplasmic domain of c-Mpl. 48 positive clones were isolated from 5 X 10(6) independent transformants. The results of sequence analysis demonstrate that they represent 13 different protein encoding sequences. Among them there are a partial coding sequence of serine/threonine protein kinase SGK (serum and glucocorticoid-inducible kinase) and 14-3-3 theta protein partial coding sequence. GST-pull-down assay and co-immunoprecipitation in mammal cells have confirmed the Interaction between these two proteins and c-Mpl. By constructing a series of deleted c-Mpl cytoplasmic domain, the interaction region in c-Mpl cytoplasmic tail was localized In amino acids 523-554. At the same time, the directed interaction between SGK and 14-3-3 proteins also has been verified by yeast two-hybrid assay. The present note is the first time to report that two proteins act with c-Mpl at the same time and put forward that SGK and 14-3-3 protein may be Involved in the serine/threonine phosphorylation mechanism for signal transduction.

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Documento generato il 23/01/20 alle ore 04:08:56